| Host: | Rabbit |
| Applications: | WB/IHC |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phosphorylase b kinase regulatory subunit alpha, liver isoform (911-961 aa) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000IHC-P 1:50-300 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PHKA2 |
| Gene ID: | 5256 |
| Uniprot ID: | KPB2_HUMAN |
| Immunogen Region: | 911-961 aa |
| Specificity: | This antibody detects endogenous levels of KPB2 at Human/Mouse |
| Immunogen: | Synthesized peptide derived from the human KPB2 at the amino acid range 911-961 |
| Post Translational Modifications | Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated. |
| Function | Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin. |
| Protein Name | Phosphorylase B Kinase Regulatory Subunit Alpha - Liver IsoformPhosphorylase Kinase Alpha L Subunit |
| Database Links | Reactome: R-HSA-70221 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic Side |
| Alternative Antibody Names | Anti-Phosphorylase B Kinase Regulatory Subunit Alpha - Liver Isoform antibodyAnti-Phosphorylase Kinase Alpha L Subunit antibodyAnti-PHKA2 antibodyAnti-PHKLA antibodyAnti-PYK antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and DNA Ligase IV knockout (KO) HeLa cells, using [KO Validated] DNA Ligase IV Rabbit pAb (STJ11106799) at 1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/185995/407755/STJ11106799_1__67131.1744894266.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and DNA Ligase IV knockout (KO) HeLa cells, using [KO Validated] DNA Ligase IV Rabbit pAb (STJ11106799) at 1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 1min.")
