• Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g PHGDH antibody (STJ112490). Western blot was performed from the immunoprecipitate using PHGDH antibody (STJ112490) at a dilution of 1:1000.
  • Immunofluorescence analysis of PC-12 cells using PHGDH Rabbit polyclonal antibody (STJ112490) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using PHGDH Rabbit polyclonal antibody (STJ112490) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using PHGDH Rabbit polyclonal antibody (STJ112490) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using PHGDH Rabbit polyclonal antibody (STJ112490) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PHGDH antibody (264-533) (STJ112490)

SKU:
STJ112490

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:100-1:500
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirement
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PHGDH
Gene ID: 26227
Uniprot ID: SERA_HUMAN
Immunogen Region: 264-533
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 264-533 of human PHGDH (NP_006614.2).
Immunogen Sequence: EEPPRDRALVDHENVISCPH LGASTKEAQSRCGEEIAVQF VDMVKGKSLTGVVNAQALTS AFSPHTKPWIGLAEALGTLM RAWAGSPKGTIQVITQGTSL KNAGNCLSPAVIVGLLKEAS KQADVNLVNAKLLVKEAGLN VTTSHSPAAPGEQGFGECLL AVALAGAPYQAVGLVQGTTP VLQGLNGAVFRPEVPLRRDL PLLLFRTQTSDPAMLPTMIG LLAEAGVRLLSYQTSLVSD
Function Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate and the reversible oxidation of (S)-malate to oxaloacetate.
Protein Name D-3-Phosphoglycerate Dehydrogenase
3-Pgdh
2-Oxoglutarate Reductase
Malate Dehydrogenase
Database Links Reactome: R-HSA-977347
Alternative Antibody Names Anti-D-3-Phosphoglycerate Dehydrogenase antibody
Anti-3-Pgdh antibody
Anti-2-Oxoglutarate Reductase antibody
Anti-Malate Dehydrogenase antibody
Anti-PHGDH antibody
Anti-PGDH3 antibody

Information sourced from Uniprot.org

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