| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PHF2 (Internal Region) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS + 0.02% NaN3 |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:500-1:1000IHCICCIP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PHF2 |
| Gene ID: |
5253 |
| Uniprot ID: |
PHF2_HUMAN |
| Immunogen Region: |
Internal Region |
| Specificity: |
Reacts with residues from an internal region of the 120 kDa PHF protein. |
| Immunogen: |
Synthetic peptide corresponding to residues from an internal region of the 120 kDa PHF protein |
| Post Translational Modifications | Phosphorylated by PKA on specific serine residues, leading to the formation of an active lysine demethylase complex. |
| Function | Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA. Involved in the activation of toll-like receptor 4 (TLR4)-target inflammatory genes in macrophages by catalyzing the demethylation of trimethylated histone H4 lysine 20 (H4K20me3) at the gene promoters. |
| Protein Name | Lysine-Specific Demethylase Phf2Grc5Phd Finger Protein 2 |
| Database Links | Reactome: R-HSA-3214842 |
| Cellular Localisation | NucleusNucleolusChromosomeCentromereKinetochore |
| Alternative Antibody Names | Anti-Lysine-Specific Demethylase Phf2 antibodyAnti-Grc5 antibodyAnti-Phd Finger Protein 2 antibodyAnti-PHF2 antibodyAnti-CENP-35 antibodyAnti-KIAA0662 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
