Anti-PHF19-Isoform b antibody (Internal) (STJ73548)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-PHF19-Isoform b (Internal) is suitable for use in ELISA research applications. |
| Applications | Pep-ELISA |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments in Human Kidney, Skeletal Muscle and Placenta lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | PHF19 |
| Gene ID | 26147 |
| Uniprot ID | PHF19_HUMAN |
| Accession Number | NP_001009936.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | DQRLPSQSLSSKQKGH |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_056466.1; NP_001009936.1). |
Additional Info
| Function | Polycomb group (PcG) protein that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex, thus enhancing PRC2 H3K27me3 methylation activity. Probably involved in the transition from an active state to a repressed state in embryonic stem cells: acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting H3K36me3 histone demethylases RIOX1 or KDM2B, leading to demethylation of H3K36 and recruitment of the PRC2 complex that mediates H3K27me3 methylation, followed by de novo silencing. Recruits the PRC2 complex to CpG islands and contributes to embryonic stem cell self-renewal. Also binds histone H3 dimethylated at 'Lys-36' (H3K36me2). Isoform 1 and isoform 2 inhibit transcription from an HSV-tk promoter. |
| Protein Name | Phd Finger Protein 19Polycomb-Like Protein 3Hpcl3 |
| Database Links | Reactome: R-HSA-212300 |
| Cellular Localisation | NucleusLocalizes To Chromatin As Part Of The Prc2 Complex |
| Alternative Antibody Names | Anti-Phd Finger Protein 19 antibodyAnti-Polycomb-Like Protein 3 antibodyAnti-Hpcl3 antibodyAnti-PHF19 antibodyAnti-PCL3 antibody |
Information sourced from Uniprot.org