• Western blot analysis of extracts from wild type (WT) and PHF11 knockdown (KD) 293T cells, using PHF11 Rabbit monoclonal antibody (STJ11105273) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of Mouse spleen, using PHF11 Rabbit monoclonal antibody (STJ11105273) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PHF11 antibody (40-321) [S5273RM] (STJ11105273)

SKU:
STJ11105273

£146.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Phd Finger Protein 11 (40-321) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5273RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PHF11
Gene ID: 51131
Uniprot ID: PHF11_HUMAN
Immunogen Region: 40-321
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 40-321 of human PHF11 (NP_001035533.1).
Immunogen Sequence: MEKRTCALCPKDVEYNVLYF AQSENIAAHENCLLYSSGLV ECEDQDPLNPDRSFDVESVK KEIQRGRKLKCKFCHKRGAT VGCDLKNCNKNYHFFCAKKD DAVPQSDGVRGIYKLLCQQH AQFPIIAQSAKFSGVKRKRG RKKPLSGNHVQPPETMKCNT FIRQVKEEHGRHTDATVKVP FLKKCKEAGLLNYLLEEILD KVHSIPEKLMDETTSESDYE EIGSALFDCRLFEDTFVNF
Tissue Specificity Highly expressed in T and B-cells, as well as natural killer and mature dendritic cells. Expressed at higher levels in Th1 as compared to Th2 cells. Expressed at low levels in all normal tissues tested, including lung, testis, small intestine, breast, liver and placenta.
Function Positive regulator of Th1-type cytokine gene expression.
Protein Name Phd Finger Protein 11
Brca1 C-Terminus-Associated Protein
Renal Carcinoma Antigen Ny-Ren-34
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Phd Finger Protein 11 antibody
Anti-Brca1 C-Terminus-Associated Protein antibody
Anti-Renal Carcinoma Antigen Ny-Ren-34 antibody
Anti-PHF11 antibody
Anti-BCAP antibody

Information sourced from Uniprot.org

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