• Western blot analysis of lysates from HeLa cells, using PHF1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using PHF1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-PHF1 antibody (345-394 aa) (STJ95062)

SKU:
STJ95062

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PHD finger protein 1 (345-394 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PHF1
Gene ID: 5252
Uniprot ID: PHF1_HUMAN
Immunogen Region: 345-394 aa
Specificity: PHF1 Polyclonal Antibody detects endogenous levels of PHF1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human PHF1 at the amino acid range 345-394
Function Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Involved in DNA damage response and is recruited at double-strand breaks (DSBs). Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex: it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. According to some reports, PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci. According to another report, PHF1 recruits the PRC2 complex at double-strand breaks (DSBs) and inhibits the activity of PRC2. Regulates p53/TP53 stability and prolonges its turnover: may act by specifically binding to a methylated from of p53/TP53.
Protein Name Phd Finger Protein 1
Protein Phf1
Hphf1
Polycomb-Like Protein 1
Hpcl1
Database Links Reactome: R-HSA-212300
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Localizes Specifically To The Promoters Of Numerous Target Genes
Localizes To Double-Strand Breaks (Dsbs) Sites Following Dna Damage
Co-Localizes With Nek6 In The Centrosome
Alternative Antibody Names Anti-Phd Finger Protein 1 antibody
Anti-Protein Phf1 antibody
Anti-Hphf1 antibody
Anti-Polycomb-Like Protein 1 antibody
Anti-Hpcl1 antibody
Anti-PHF1 antibody
Anti-PCL1 antibody

Information sourced from Uniprot.org

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