| Host: |
Rabbit |
| Applications: |
WB/IHC/IP |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PHB2 (1-299) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:100IP 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PHB2 |
| Gene ID: |
11331 |
| Uniprot ID: |
PHB2_HUMAN |
| Immunogen Region: |
1-299 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-299 of human Prohibitin 2 (Prohibitin 2 (PHB2) ) (NP_001138303.1). |
| Immunogen Sequence: |
MAQNLKDLAGRLPAGPRGMG TALKLLLGAGAVAYGVRESV FTVEGGHRAIFFNRIGGVQQ DTILAEGLHFRIPWFQYPII YDIRARPRKISSPTGSKDLQ MVNISLRVLSRPNAQELPSM YQRLGLDYEERVLPSIVNEV LKSVVAKFNASQLITQRAQV SLLIRRELTERAKDFSLILD DVAITELSFSREYTAAVEAK QVAQQEAQRAQFLVEKAKQE QRQKIVQAEGEAEAAKMLG |
| Post Translational Modifications | Phosphorylated. Tyrosine phosphorylation is indirectly stimulated by IGFBP6. |
| Function | Protein with pleiotropic attributes mediated in a cell-compartment- and tissue-specific manner, which include the plasma membrane-associated cell signaling functions, mitochondrial chaperone, and transcriptional co-regulator of transcription factors and sex steroid hormones in the nucleus. In the mitochondria, together with PHB, forms large ring complexes (prohibitin complexes) in the inner mitochondrial membrane (IMM) and functions as chaperone protein that stabilizes mitochondrial respiratory enzymes and maintains mitochondrial integrity in the IMM, which is required for mitochondrial morphogenesis, neuronal survival, and normal lifespan (Probable). The prohibitin complex, with DNAJC19, regulates cardiolipin remodeling and the protein turnover of OMA1 in a cardiolipin-binding manner. Also regulates cytochrome-c oxidase assembly (COX) and mitochondrial respiration. Binding to sphingoid 1-phosphate (SPP) modulates its regulator activity. Has a key role of mitophagy receptor involved in targeting mitochondria for autophagic degradation. Involved in mitochondrial-mediated antiviral innate immunity, activates RIG-I-mediated signal transduction and production of IFNB1 and pro-inflammatory cytokine IL6. In the nucleus, serves as transcriptional co-regulator (Probable). Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. In the plasma membrane, is involved in IGFBP6-induced cell migration. Cooperates with CD86 to mediate CD86-signaling in B lymphocytes that regulates the level of IgG1 produced through the activation of distal signaling intermediates. Upon CD40 engagement, required to activate NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation. (Microbial infection) Involved in human enterovirus 71/EV-71 infection by enhancing the autophagy mechanism during the infection. |
| Protein Name | Prohibitin-2B-Cell Receptor-Associated Protein Bap37D-ProhibitinRepressor Of Estrogen Receptor Activity |
| Database Links | Reactome: R-HSA-8949664 |
| Cellular Localisation | Mitochondrion Inner MembraneCytoplasmNucleusCell MembraneIsoform 1: Mitochondrion Inner MembraneIsoform 2: Mitochondrion Inner Membrane |
| Alternative Antibody Names | Anti-Prohibitin-2 antibodyAnti-B-Cell Receptor-Associated Protein Bap37 antibodyAnti-D-Prohibitin antibodyAnti-Repressor Of Estrogen Receptor Activity antibodyAnti-PHB2 antibodyAnti-BAP antibodyAnti-REA antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and NHEJ1 knockout (KO) HeLa cells, using [KO Validated] NHEJ1 Rabbit polyclonal antibody (STJ11103937) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/176582/367429/STJ11103937_1__73085.1713132967.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NHEJ1 knockout (KO) HeLa cells, using [KO Validated] NHEJ1 Rabbit polyclonal antibody (STJ11103937) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.")