Anti-PHB antibody (73-272) (STJ115487)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PHB (73-272) antibody for use in WB, IHC-P and ELISA in human, mouse, rat and monkey samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat/Monkey |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:100-1:500ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PHB1 |
| Gene ID | 5245 |
| Uniprot ID | PHB1_HUMAN |
| Immunogen Region | 73-272 |
| Immunogen Sequence | PRNVPVITGSKDLQNVNITL RILFRPVASQLPRIFTSIGE DYDERVLPSITTEILKSVVA RFDAGELITQRELVSRQVSD DLTERAATFGLILDDVSLTH LTFGKEFTEAVEAKQVAQQE AERARFVVEKAEQQKKAAII SAEGDSKAAELIANSLATAG DGLIELRKLEAAEDIAYQLS RSRNITYLPAGQSVLLQLPQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 73-272 of human Prohibitin (NP_002625.1). |
Additional Info
| Tissue Specificity | Widely expressed in different tissues. |
| Function | Protein with pleiotropic attributes mediated in a cell-compartment- and tissue-specific manner, which include the plasma membrane-associated cell signaling functions, mitochondrial chaperone, and transcriptional co-regulator of transcription factors in the nucleus. Plays a role in adipose tissue and glucose homeostasis in a sex-specific manner. Contributes to pulmonary vascular remodeling by accelerating proliferation of pulmonary arterial smooth muscle cells. In the mitochondria, together with PHB2, forms large ring complexes (prohibitin complexes) in the inner mitochondrial membrane (IMM) and functions as a chaperone protein that stabilizes mitochondrial respiratory enzymes and maintains mitochondrial integrity in the IMM, which is required for mitochondrial morphogenesis, neuronal survival, and normal lifespan (Probable). The prohibitin complex, with DNAJC19, regulates cardiolipin remodeling and the protein turnover of OMA1 in a cardiolipin-binding manner. Regulates mitochondrial respiration activity playing a role in cellular aging. The prohibitin complex plays a role of mitophagy receptor involved in targeting mitochondria for autophagic degradation. Involved in mitochondrial-mediated antiviral innate immunity, activates RIG-I-mediated signal transduction and production of IFNB1 and pro-inflammatory cytokine IL6. In the nucleus, acts as a transcription coregulator, enhances promoter binding by TP53, a transcription factor it activates, but reduces the promoter binding by E2F1, a transcription factor it represses. Interacts with STAT3 to affect IL17 secretion in T-helper Th17 cells. In the plasma membrane, cooperates with CD86 to mediate CD86-signaling in B lymphocytes that regulates the level of IgG1 produced through the activation of distal signaling intermediates. Upon CD40 engagement, required to activate NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation. |
| Protein Name | Prohibitin 1 |
| Database Links | Reactome: R-HSA-5673000Reactome: R-HSA-6802946Reactome: R-HSA-6802955Reactome: R-HSA-8949664Reactome: R-HSA-9649948 |
| Cellular Localisation | Mitochondrion Inner MembraneNucleusCytoplasmCell Membrane |
| Alternative Antibody Names | Anti-Prohibitin 1 antibodyAnti-PHB1 antibodyAnti-PHB antibody |
Information sourced from Uniprot.org