Anti-PGRMC1 antibody (44-195) (STJ27586)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PGRMC1 (44-195) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:100IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PGRMC1 |
| Gene ID | 10857 |
| Uniprot ID | PGRC1_HUMAN |
| Immunogen Region | 44-195 |
| Immunogen Sequence | KIVRGDQPAASGDSDDDEPP PLPRLKRRDFTPAELRRFDG VQDPRILMAINGKVFDVTKG RKFYGPEGPYGVFAGRDASR GLATFCLDKEALKDEYDDLS DLTAAQQETLSDWESQFTFK YHHVGKLLKEGEEPTVYSDE EEPKDESARKND |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 44-195 of human PGRMC1 (NP_006658.1). |
Additional Info
| Tissue Specificity | Detected in urine (at protein level). Expressed by endometrial glands and stroma (at protein level). Widely expressed, with highest expression in liver and kidney. |
| Post Translational Modifications | O-glycosylated.contains chondroitin sulfate attached to Ser-54. Ser-54 is in the cytoplasmic domain but the glycosylated form was detected in urine, suggesting that the membrane-bound form is cleaved, allowing for production of a secreted form which is glycosylated. |
| Function | Component of a progesterone-binding protein complex. Binds progesterone. Has many reported cellular functions (heme homeostasis, interaction with CYPs). Required for the maintenance of uterine histoarchitecture and normal female reproductive lifespan. Intracellular heme chaperone. Regulates heme synthesis via interactions with FECH and acts as a heme donor for at least some hemoproteins. Forms a ternary complex with TMEM97 receptor and low density lipid receptor/LDLR, which increases LDLR-mediated LDL lipoprotein internalization. |
| Protein Name | Membrane-Associated Progesterone Receptor Component 1MprDap1Iza |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | Microsome MembraneSingle-Pass Membrane ProteinSmooth Endoplasmic Reticulum MembraneMitochondrion Outer MembraneExtracellular SideSecretedLocalized At Cell MembraneProbably In Lipid RaftsIn Serum-Starved Conditions |
| Alternative Antibody Names | Anti-Membrane-Associated Progesterone Receptor Component 1 antibodyAnti-Mpr antibodyAnti-Dap1 antibodyAnti-Iza antibodyAnti-PGRMC1 antibodyAnti-HPR6.6 antibodyAnti-PGRMC antibody |
Information sourced from Uniprot.org