• Immunofluorescence analysis of L929 cells using PGK2 Rabbit polyclonal antibody (STJ114818) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using PGK2 Rabbit polyclonal antibody (STJ114818) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of PGK2 in paraffin-embedded mouse testis using PGK2 Rabbit polyclonal antibody (STJ114818) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PGK2 in paraffin-embedded rat testis using PGK2 Rabbit polyclonal antibody (STJ114818) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PGK2 in paraffin-embedded human mammary cancer using PGK2 Rabbit polyclonal antibody (STJ114818) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using PGK2 Rabbit polyclonal antibody (STJ114818) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-PGK2 antibody (242-360) (STJ114818)

SKU:
STJ114818

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:100
IF/ICC:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PGK2
Gene ID: 5232
Uniprot ID: PGK2_HUMAN
Immunogen Region: 242-360
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 242-360 of human PGK2 (NP_620061.2).
Immunogen Sequence: YTFLKVLNNMEIGASLFDEE GAKIVKDIMAKAQKNGVRIT FPVDFVTGDKFDENAQVGKA TVASGISPGWMGLDCGPESN KNHAQVVAQARLIVWNGPLG VFEWDAFAKGTKALMDEIV
Tissue Specificity Mainly found in round spermatids. Localized on the principle piece in the sperm (at protein level). Testis-specific. Expression significantly decreased in the testis of elderly men.
Function Essential for sperm motility and male fertility. Not required for the completion of spermatogenesis.
Protein Name Phosphoglycerate Kinase 2
Phosphoglycerate Kinase - Testis Specific
Database Links Reactome: R-HSA-70171
Reactome: R-HSA-70263
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Phosphoglycerate Kinase 2 antibody
Anti-Phosphoglycerate Kinase - Testis Specific antibody
Anti-PGK2 antibody
Anti-PGKB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance