• Immunoprecipitation analysis of 300 Mu g extracts of Jurkat cells using 3 Mu g PGD antibody (STJ11102692). Western blot was performed from the immunoprecipitate using PGD antibody (STJ11102692) at a dilution of 1:1000.
  • Immunofluorescence analysis of PC-12 cells using PGD Rabbit monoclonal antibody (STJ11102692) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using PGD Rabbit monoclonal antibody (STJ11102692) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using PGD Rabbit monoclonal antibody (STJ11102692) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-PGD antibody (384-483) [S2MR] (STJ11102692)

SKU:
STJ11102692

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PGD (384-483) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PGD
Gene ID: 5226
Uniprot ID: 6PGD_HUMAN
Immunogen Region: 384-483
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 384-483 of human PGD (P52209).
Immunogen Sequence: PELQNLLLDDFFKSAVENCQ DSWRRAVSTGVQAGIPMPCF TTALSFYDGYRHEMLPASLI QAQRDYFGAHTYELLAKPGQ FIHTNWTGHGGTVSSSSYNA
Function Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.
Protein Name 6-Phosphogluconate Dehydrogenase - Decarboxylating
Database Links Reactome: R-HSA-71336
Reactome: R-HSA-9818028
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-6-Phosphogluconate Dehydrogenase - Decarboxylating antibody
Anti-PGD antibody
Anti-PGDH antibody

Information sourced from Uniprot.org

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