| Host: | Goat |
| Applications: | Pep-ELISA/IF/IHC/FC |
| Reactivity: | Human/Mouse/Rat/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-PGC1A/PPARGC1A (Internal) is suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Flow Cytometry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:2000. WB: Preliminary testing showed a band at approx 90kDa in nuclear lysates of cell lines A431 and HEK293 after 0.3-1µg/ml antibody staining (calculated MW of 91.0kDa according to Human NP_037 |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | PPARGC1A |
| Gene ID: | 10891 |
| Uniprot ID: | PRGC1_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_037393.1; NP_001317680.1; NP_001317681.1; NP_001317682.1; NP_001341756.1;Isotype=IgG" |
| Immunogen Sequence: | DGLFDDSEDESDK |
| Post Translational Modifications | Phosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2. Heavily acetylated by KAT2A/GCN5 under conditions of high nutrients, leading to inactivation of PPARGC1A. Deacetylated by SIRT1 in low nutrients/high NAD conditions, leading to its activation. Ubiquitinated. Ubiquitination by RNF34 induces proteasomal degradation. |
| Function | Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Acts as a key regulator of gluconeogenesis: stimulates hepatic gluconeogenesis by increasing the expression of gluconeogenic enzymes, and acting together with FOXO1 to promote the fasting gluconeogenic program. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK. |
| Protein Name | Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-Alpha Pgc-1-Alpha Ppar-Gamma Coactivator 1-Alpha Ppargc-1-Alpha Ligand Effect Modulator 6 |
| Database Links | Reactome: R-HSA-1989781 Reactome: R-HSA-2151201 Reactome: R-HSA-2151209 Reactome: R-HSA-381340 Reactome: R-HSA-3899300 Reactome: R-HSA-400253 Reactome: R-HSA-8939902 Reactome: R-HSA-9615017 Reactome: R-HSA-9707616 Reactome: R-HSA-9844594 Reactome: R-HSA-9854907 |
| Cellular Localisation | Isoform 1: Nucleus Nucleus Pml Body Isoform B4: Nucleus Isoform B4-8a: Cytoplasm Isoform B5: Nucleus Isoform 9: Nucleus |
| Alternative Antibody Names | Anti-Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-Alpha antibody Anti-Pgc-1-Alpha antibody Anti-Ppar-Gamma Coactivator 1-Alpha antibody Anti-Ppargc-1-Alpha antibody Anti-Ligand Effect Modulator 6 antibody Anti-PPARGC1A antibody Anti-LEM6 antibody Anti-PGC1 antibody Anti-PGC1A antibody Anti-PPARGC1 antibody |
Information sourced from Uniprot.org