| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Dog/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-PGAM1/PGAM2/PGAM4 (C-Term) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 28kDa band observed in Human, Mouse, Rat and Pig Liver lysates (calculated MW of 28.8kDa according to NP_002620.1; NP_000281.2 and of NP_001025062.1). The additional 30kDa band |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | PGAM1 |
| Gene ID: | 5223 |
| Uniprot ID: | PGAM1_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_002620.1; NP_000281.2; NP_001025062.1 |
| Specificity: | Please note this antibody is expected to recognize the products of 3 highly similar genes. |
| Immunogen Sequence: | KAMEAVAAQGKAKK |
| Post Translational Modifications | Acetylated at Lys-253, Lys-253 and Lys-254 under high glucose condition. Acetylation increases catalytic activity. Under glucose restriction SIRT1 levels dramatically increase and it deacetylates the enzyme. |
| Function | Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglyceratea crucial step in glycolysis, by using 2,3-bisphosphoglycerate. Also catalyzes the interconversion of (2R)-2,3-bisphosphoglycerate and (2R)-3-phospho-glyceroyl phosphate. |
| Protein Name | Phosphoglycerate Mutase 1Bpg-Dependent Pgam 1Phosphoglycerate Mutase Isozyme BPgam-B |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-70171Reactome: R-HSA-70263 |
| Alternative Antibody Names | Anti-Phosphoglycerate Mutase 1 antibodyAnti-Bpg-Dependent Pgam 1 antibodyAnti-Phosphoglycerate Mutase Isozyme B antibodyAnti-Pgam-B antibodyAnti-PGAM1 antibodyAnti-PGAMA antibodyAnti-CDABP0006 antibody |
Information sourced from Uniprot.org