Host: | Rabbit |
Applications: | WB/ICC |
Reactivity: | Human/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Rabbit polyclonal antibody anti-Profilin (C-Term) is suitable for use in Western Blot and Immunocytochemistry research applications. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
Purification: | Antigen Affinity Purified |
Dilution Range: | WB 1:1000 IHC ICC 1:100 IP |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | PFN1 |
Gene ID: | 5216 |
Uniprot ID: | PROF1_HUMAN |
Immunogen Region: | C-Term |
Specificity: | The antibody detects a 15 kDa band corresponding to the apparent molecular mass of Profilin-1 in human umbilical vein endothelial cells (HUVEC) and Jurkat cells. |
Immunogen: | Profilin synthetic peptide (coupled to carrier protein) that includes amino acids from the C-terminal region of human Profilin-1. This sequence is well conserved in rat and mouse Profilin-1, has some homology to Profilin-2a and Profilin-2b, and low h |
Post Translational Modifications | Phosphorylation at Ser-138 reduces its affinity for G-actin and blocks its interaction with HTT, reducing its ability to inhibit androgen receptor (AR) and HTT aggregation. |
Function | Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR. |
Protein Name | Profilin-1 Epididymis Tissue Protein Li 184a Profilin I |
Database Links | Reactome: R-HSA-114608 Reactome: R-HSA-376176 Reactome: R-HSA-4086400 Reactome: R-HSA-5663220 |
Cellular Localisation | Cytoplasm Cytoskeleton |
Alternative Antibody Names | Anti-Profilin-1 antibody Anti-Epididymis Tissue Protein Li 184a antibody Anti-Profilin I antibody Anti-PFN1 antibody |
Information sourced from Uniprot.org