• Western blot analysis of extracts of various cell lines, using Profilin1 Polyclonal Antibody (STJ24964) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of various cell lines, using Profilin1 Polyclonal Antibody (STJ24964) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PFN1 antibody (1-140) (STJ24964)

SKU:
STJ24964

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PFN1 (1-140) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PFN1
Gene ID: 5216
Uniprot ID: PROF1_HUMAN
Immunogen Region: 1-140
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human Profilin1 (NP_005013.1).
Immunogen Sequence: MAGWNAYIDNLMADGTCQDA AIVGYKDSPSVWAAVPGKTF VNITPAEVGVLVGKDRSSFY VNGLTLGGQKCSVIRDSLLQ DGEFSMDLRTKSTGGAPTFN VTVTKTDKTLVLLMGKEGVH GGLINKKCYEMASHLRRSQY
Tissue Specificity Expressed in epididymis (at protein level).
Post Translational Modifications Phosphorylation at Ser-138 reduces its affinity for G-actin and blocks its interaction with HTT, reducing its ability to inhibit androgen receptor (AR) and HTT aggregation.
Function Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.
Protein Name Profilin-1
Epididymis Tissue Protein Li 184a
Profilin I
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-376176
Reactome: R-HSA-4086400
Reactome: R-HSA-5663220
Cellular Localisation Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Profilin-1 antibody
Anti-Epididymis Tissue Protein Li 184a antibody
Anti-Profilin I antibody
Anti-PFN1 antibody

Information sourced from Uniprot.org

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