• Immunofluorescence analysis of U-2 OS cells using PFKM Rabbit polyclonal antibody (STJ27430) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using PFKM Rabbit polyclonal antibody (STJ27430) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using PFKM Rabbit polyclonal antibody (STJ27430) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using PFKM Rabbit polyclonal antibody (STJ27430) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using PFKM antibody (STJ27430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PFKM antibody (501-780) (STJ27430)

SKU:
STJ27430

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PFKM (501-780) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PFKM
Gene ID: 5213
Uniprot ID: PFKAM_HUMAN
Immunogen Region: 501-780
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 501-780 of human PFKM (NP_001160160.1).
Immunogen Sequence: AYTGGLELMEGRKQFDELCI PFVVIPATVSNNVPGSDFSV GADTALNTICTTCDRIKQSA AGTKRRVFIIETMGGYCGYL ATMAGLAAGADAAYIFEEPF TIRDLQANVEHLVQKMKTTV KRGLVLRNEKCNENYTTDFI FNLYSEEGKGIFDSRKNVLG HMQQGGSPTPFDRNFATKMG AKAMNWMSGKIKESYRNGRI FANTPDSGCVLGMRKRALVF QPVAELKDQTDFEHRIPKE
Post Translational Modifications GlcNAcylation decreases enzyme activity.
Function Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Protein Name Atp-Dependent 6-Phosphofructokinase - Muscle Type
Atp-Pfk
Pfk-M
6-Phosphofructokinase Type A
Phosphofructo-1-Kinase Isozyme A
Pfk-A
Phosphohexokinase
Database Links Reactome: R-HSA-70171
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Atp-Dependent 6-Phosphofructokinase - Muscle Type antibody
Anti-Atp-Pfk antibody
Anti-Pfk-M antibody
Anti-6-Phosphofructokinase Type A antibody
Anti-Phosphofructo-1-Kinase Isozyme A antibody
Anti-Pfk-A antibody
Anti-Phosphohexokinase antibody
Anti-PFKM antibody
Anti-PFKX antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance