• Western blot analysis of extracts of various cell lines, using PFKFB2 antibody (STJ111650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of L929 cells using PFKFB2 Rabbit polyclonal antibody (STJ111650) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using PFKFB2 rabbit polyclonal antibody (STJ111650) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using PFKFB2 Rabbit polyclonal antibody (STJ111650) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using PFKFB2 rabbit polyclonal antibody (STJ111650) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using PFKFB2 antibody (STJ111650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PFKFB2 antibody (446-505) (STJ111650)

SKU:
STJ111650

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PFKFB2 (446-505) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PFKFB2
Gene ID: 5208
Uniprot ID: F262_HUMAN
Immunogen Region: 446-505
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 446-505 of human PFKFB2 (NP_006203.2).
Immunogen Sequence: RDKPTNNFPKNQTPVRMRRN SFTPLSSSNTIRRPRNYSVG SRPLKPLSPLRAQDMQEGAD
Tissue Specificity Heart.
Post Translational Modifications Phosphorylation by AMPK stimulates activity.
Function Synthesis and degradation of fructose 2,6-bisphosphate.
Protein Name 6-Phosphofructo-2-Kinase/Fructose-2 -6-Bisphosphatase 2
6pf-2-K/Fru-2 -6-P2ase 2
Pfk/Fbpase 2
6pf-2-K/Fru-2 -6-P2ase Heart-Type Isozyme Includes - 6-Phosphofructo-2-Kinase - Fructose-2 -6-Bisphosphatase
Database Links Reactome: R-HSA-9634600
Alternative Antibody Names Anti-6-Phosphofructo-2-Kinase/Fructose-2 -6-Bisphosphatase 2 antibody
Anti-6pf-2-K/Fru-2 -6-P2ase 2 antibody
Anti-Pfk/Fbpase 2 antibody
Anti-6pf-2-K/Fru-2 -6-P2ase Heart-Type Isozyme Includes - 6-Phosphofructo-2-Kinase - Fructose-2 -6-Bisphosphatase antibody
Anti-PFKFB2 antibody

Information sourced from Uniprot.org

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