• Western blot analysis of mouse kidney, using PEX14 rabbit polyclonal antibody (STJ29475) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of A-431, using PEX14 rabbit polyclonal antibody (STJ29475) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human prostate cancer using PEX14 antibody (STJ29475) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using PEX14 antibody (STJ29475) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PEX14 antibody (118-377) (STJ29475)

SKU:
STJ29475

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PEX14 (118-377) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PEX14
Gene ID: 5195
Uniprot ID: PEX14_HUMAN
Immunogen Region: 118-377
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 118-377 of human PEX14 (NP_004556.1).
Immunogen Sequence: GIAFGFHQLYKKYLLPLILG GREDRKQLERMEAGLSELSG SVAQTVTQLQTTLASVQELL IQQQQKIQELAHELAAAKAT TSTNWILESQNINELKSEIN SLKGLLLNRRQFPPSPSAPK IPSWQIPVKSPSPSSPAAVN HHSSSDISPVSNESTSSSPG KEGHSPEGSTVTYHLLGPQE EGEGVVDVKGQVRMEVQGEE EKREDKEDEEDEEDDDVSHV DEEDCLGVQREDRRGGDGQ
Function Component of the PEX13-PEX14 docking complex, a translocon channel that specifically mediates the import of peroxisomal cargo proteins bound to PEX5 receptor. The PEX13-PEX14 docking complex forms a large import pore which can be opened to a diameter of about 9 nm. Mechanistically, PEX5 receptor along with cargo proteins associates with the PEX14 subunit of the PEX13-PEX14 docking complex in the cytosol, leading to the insertion of the receptor into the organelle membrane with the concomitant translocation of the cargo into the peroxisome matrix. Plays a key role for peroxisome movement through a direct interaction with tubulin.
Protein Name Peroxisomal Membrane Protein Pex14
Pts1 Receptor-Docking Protein
Peroxin-14
Peroxisomal Membrane Anchor Protein Pex14
Database Links Reactome: R-HSA-8866654
Reactome: R-HSA-9033241
Reactome: R-HSA-9603798
Cellular Localisation Peroxisome Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Peroxisomal Membrane Protein Pex14 antibody
Anti-Pts1 Receptor-Docking Protein antibody
Anti-Peroxin-14 antibody
Anti-Peroxisomal Membrane Anchor Protein Pex14 antibody
Anti-PEX14 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance