| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PEX10 (10-160) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:5000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PEX10 |
| Gene ID: |
5192 |
| Uniprot ID: |
PEX10_HUMAN |
| Immunogen Region: |
10-160 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 10-160 of human PEX10 (NP_002608.1). |
| Immunogen Sequence: |
EVIRAAQKDEYYRGGLRSAA GGALHSLAGARKWLEWRKEV ELLSDVAYFGLTTLAGYQTL GEEYVSIIQVDPSRIHVPSS LRRGVLVTLHAVLPYLLDKA LLPLEQELQADPDSGRPLQG SLGPGGRGCSGARRWMRHHT ATLTEQQRRAL |
| Function | E3 ubiquitin-protein ligase component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling. The retrotranslocation channel is composed of PEX2, PEX10 and PEX12.each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 through the peroxisomal membrane. PEX10 also regulates PEX5 recycling by acting as a E3 ubiquitin-protein ligase. When PEX5 recycling is compromised, PEX10 catalyzes polyubiquitination of PEX5 during its passage through the retrotranslocation channel, leading to its degradation. |
| Protein Name | Peroxisome Biogenesis Factor 10Peroxin-10Peroxisomal Biogenesis Factor 10Peroxisome Assembly Protein 10Ring Finger Protein 69 |
| Database Links | Reactome: R-HSA-8866654Reactome: R-HSA-9033241 |
| Cellular Localisation | Peroxisome MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Peroxisome Biogenesis Factor 10 antibodyAnti-Peroxin-10 antibodyAnti-Peroxisomal Biogenesis Factor 10 antibodyAnti-Peroxisome Assembly Protein 10 antibodyAnti-Ring Finger Protein 69 antibodyAnti-PEX10 antibodyAnti-RNF69 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and LYAR knockout (KO) 293T cells, using [KO Validated] LYAR Rabbit polyclonal antibody (STJ11100776) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89712/358194/STJ11100776_1__10553.1713122510.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and LYAR knockout (KO) 293T cells, using [KO Validated] LYAR Rabbit polyclonal antibody (STJ11100776) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
