Anti-Peroxiredoxin-6 antibody (STJA0041032)
SPECIFICATIONS
ClonalityPolyclonal
HostSheep
ConjugationUnconjugated
IsotypeMixed
ImmunogenRat recombinant Peroxiredoxin-6
General Information
| Short Description | Sheep polyclonal anti-Peroxiredoxin-6 for use in ELISA, IHC-F, IHC-P and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/IHC-F/IHC-P/WB |
| Host | Sheep |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | Mixed |
| Conjugation | Unconjugated |
| Purification | Whole serum |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:1000 |
| Storage Instruction | Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at-20°C for a higher stability, and at 2-8°C with an appropriate antibacterial |
Target Information
| Gene Symbol | Prdx6 |
| Gene ID | 94167 |
| Uniprot ID | PRDX6_RAT |
| Immunogen | Rat recombinant Peroxiredoxin-6 |
| Immunogen Sequence | Rat |
| Specificity | This antibody has been shown to be specific for Peroxiredoxin-6 protein. Rat, human and mouse, other species have not yet been tested. |
Additional Info
| Post Translational Modifications | Phosphorylation at Thr-177 by MAP kinases increases the phospholipase activity of the enzyme. Phosphorylated form exhibits a greater lysophosphatidylcholine acyltransferase activity compared to the non-phosphorylated form. Irreversibly inactivated by overoxidation of Cys-47 to sulfinic acid (Cys-SO(2)H) and sulfonic acid (Cys-SO(3)H) forms upon oxidative stress. |
| Function | Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. Also has phospholipase activity, can therefore either reduce the oxidized sn-2 fatty acyl group of phospholipids (peroxidase activity) or hydrolyze the sn-2 ester bond of phospholipids (phospholipase activity). These activities are dependent on binding to phospholipids at acidic pH and to oxidized phospholipds at cytosolic pH. Plays a role in cell protection against oxidative stress by detoxifying peroxides and in phospholipid homeostasis. Exhibits acyl-CoA-dependent lysophospholipid acyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC). Shows a clear preference for LPC as the lysophospholipid and for palmitoyl CoA as the fatty acyl substrate. |
| Protein Name | Peroxiredoxin-61-Cys Peroxiredoxin1-Cys PrxAcidic Calcium-Independent Phospholipase A2Aipla2Antioxidant Protein 2Glutathione-Dependent PeroxiredoxinLysophosphatidylcholine Acyltransferase 5Lpc Acyltransferase 5Lpcat-5Lyso-Pc Acyltransferase 5Non-Selenium Glutathione PeroxidaseNsgpxThiol-Specific Antioxidant Protein |
| Database Links | Reactome: R-RNO-3299685Reactome: -RNO-6798695 |
| Cellular Localisation | CytoplasmLysosomeAlso Found In Lung Secretory Organelles (Lamellar Bodies) |
| Alternative Antibody Names | Anti-Peroxiredoxin-6 antibodyAnti-1-Cys Peroxiredoxin antibodyAnti-1-Cys Prx antibodyAnti-Acidic Calcium-Independent Phospholipase A2 antibodyAnti-Aipla2 antibodyAnti-Antioxidant Protein 2 antibodyAnti-Glutathione-Dependent Peroxiredoxin antibodyAnti-Lysophosphatidylcholine Acyltransferase 5 antibodyAnti-Lpc Acyltransferase 5 antibodyAnti-Lpcat-5 antibodyAnti-Lyso-Pc Acyltransferase 5 antibodyAnti-Non-Selenium Glutathione Peroxidase antibodyAnti-Nsgpx antibodyAnti-Thiol-Specific Antioxidant Protein antibodyAnti-Prdx6 antibodyAnti-Aipla2 antibodyAnti-Aop2 antibodyAnti-Tsa antibody |
Information sourced from Uniprot.org