• Immunofluorescence analysis of paraffin-embedded Rat heart tissue using PENK Rabbit polyclonal antibody (STJ11105654) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunofluorescence analysis of paraffin-embedded Human adrenal gland tissue using PENK Rabbit polyclonal antibody (STJ11105654) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of lysates from THP-1 cells using PENK Rabbit polyclonal antibody (STJ11105654) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:90s.

Anti-PENK antibody (150-267) (STJ11105654)

SKU:
STJ11105654

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Proenkephalin-A Cleaved Into-Synenkephalin-Met-Enkephalin (150-267) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PENK
Gene ID: 5179
Uniprot ID: PENK_HUMAN
Immunogen Region: 150-267
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 150-267 of human PENK (NP_001129162.1).
Immunogen Sequence: LANSSDLLKELLETGDNRER SHHQDGSDNEEEVSKRYGGF MRGLKRSPQLEDEAKELQKR YGGFMRRVGRPEWWMDYQKR YGGFLKRFAEALPSDEEGES YSKEVPEMEKRYGGFMRF
Post Translational Modifications Proenkephalin-A is cleaved by CTSL to generate Met-enkephalin. Met-enkephalin: Processed and degraded by ACE. Leu-enkephalin: Processed and degraded by ACE. Met-enkephalin-Arg-Gly-Leu: Probably cleaved by ACE. Met-enkephalin-Arg-Phe: Processed by ACE to generate Met-enkephalin in the nucleus accumbens of the brain. The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
Function Met-enkephalin: Neuropeptide that competes with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. Leu-enkephalin: Neuropeptide that competes with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. Met-enkephalin-Arg-Phe: Met-enkephalin-Arg-Phe neuropeptide acts as a strong ligand of Mu-type opioid receptor OPRM1. Met-enkephalin-Arg-Phe-binding to OPRM1 in the nucleus accumbens of the brain increases activation of OPRM1, leading to long-term synaptic depression of glutamate release. PENK(114-133): Increases glutamate release in the striatum and decreases GABA concentration in the striatum. PENK(237-258): Increases glutamate release in the striatum.
Protein Name Proenkephalin-A Cleaved Into - Synenkephalin - Met-Enkephalin
Opioid Growth Factor
Ogf - Penk(114-133 - Penk(143-183 - Met-Enkephalin-Arg-Gly-Leu - Leu-Enkephalin - Penk(237-258 - Met-Enkephalin-Arg-Phe
Database Links Reactome: R-HSA-375276
Reactome: R-HSA-381426
Reactome: R-HSA-418594
Reactome: R-HSA-8957275
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle
Chromaffin Granule Lumen
Secreted
Alternative Antibody Names Anti-Proenkephalin-A Cleaved Into - Synenkephalin - Met-Enkephalin antibody
Anti-Opioid Growth Factor antibody
Anti-Ogf - Penk(114-133 - Penk(143-183 - Met-Enkephalin-Arg-Gly-Leu - Leu-Enkephalin - Penk(237-258 - Met-Enkephalin-Arg-Phe antibody
Anti-PENK antibody

Information sourced from Uniprot.org

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