Anti-PEMT antibody (STJA0004853)

SKU:
STJA0004853

Current Stock:
Host: Rabbit
Applications: WB/IHC/ELISA
Reactivity: Human/Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phosphatidylethanolamine N-Methyltransferase is suitable for use in Western Blot, Immunohistochemistry and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Purification: Antigen affinity purification
Concentration: 1.56 mg/mL
Dilution Range: WB 1:1000-1:5000, IHC 1:50-1:300
Storage Instruction: Store at-20°C for long term, and avoid freeze-thaw cycles.
Gene Symbol: PEMT
Gene ID: 10400
Uniprot ID: PEMT_HUMAN
Immunogen: Synthetic peptide of human PEMT
Tissue Specificity Primarily expressed in liver (at protein level).
Post Translational Modifications Isoform 2 is N-glycosylated with high-mannose oligosaccharides.
Function Catalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure. Uses S-adenosylmethionine (S-adenosyl-L-methionine, SAM or AdoMet) as the methyl group donor for the methylation of phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine, PE) to phosphatidylmonomethylethanolamine (1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine, PMME), PMME to phosphatidyldimethylethanolamine (1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine, PDME), and PDME to phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine, PC), producing S-adenosyl-L-homocysteine in each step. Responsible for approximately 30% of hepatic PC with the CDP-choline pathway accounting for the other 70% (Probable). Isoform 1: Catalyzes the three sequential steps of the methylation of 1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine (PMME) to 1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine (PDME) more efficiently than isoform 2. Induces increase in PC species with longer polyunsaturated chains than isoform 2. Isoform 2: Produces a higher increase in the level of PC species containing long chains with three double bonds than isoform 1.
Protein Name Phosphatidylethanolamine N-Methyltransferase
Peamt
Pemt
Pemt2
Phospholipid Methyltransferase
Plmt
Database Links Reactome: R-HSA-1483191
Cellular Localisation Endoplasmic Reticulum
Localized In The Endoplasmic Reticulum (Er) Of The Liver And In A Lipid Metabolism-Rich Region Of The Er Known As Mitochondria-Associated Membranes
Adopts A Topography Within The Er Membrane That Positions Both Termini In The Cytosol
Isoform 1: Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Mitochondrion Membrane
Found In Endoplasmic Reticulum Where Most Pemt Activity Is Generated And In Mitochondria
Isoform 2: Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-Phosphatidylethanolamine N-Methyltransferase antibody
Anti-Peamt antibody
Anti-Pemt antibody
Anti-Pemt2 antibody
Anti-Phospholipid Methyltransferase antibody
Anti-Plmt antibody
Anti-PEMT antibody
Anti-PEMPT antibody
Anti-PNMT antibody

Information sourced from Uniprot.org

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