Anti-PEF1 antibody (109-159 aa) (STJ195013)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human PEF1 at the amino acid range 109-159
General Information
| Short Description | Rabbit polyclonal anti-Peflin (109-159 aa) for use in WB and IHC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:50-300 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PEF1 |
| Gene ID | 553115 |
| Uniprot ID | PEF1_HUMAN |
| Immunogen | Synthesized peptide derived from the human PEF1 at the amino acid range 109-159 |
| Immunogen Region | 109-159 aa |
| Specificity | This antibody detects endogenous levels of PEF1 at Human/Mouse/Rat |
Additional Info
| Function | Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as a calcium-dependent adapter for the BCR(KLHL12) complex, a complex involved in endoplasmic reticulum (ER)-Golgi transport by regulating the size of COPII coats. In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification. Its role in the heterodimer formed with PDCD6 is however unclear: some evidence shows that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium. Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6. Also acts as a negative regulator of ER-Golgi transport.possibly by inhibiting interaction between PDCD6 and SEC31. |
| Protein Name | PeflinPef Protein With A Long N-Terminal Hydrophobic DomainPenta-Ef Hand Domain-Containing Protein 1 |
| Database Links | |
| Cellular Localisation | CytoplasmEndoplasmic ReticulumMembranePeripheral Membrane ProteinCytoplasmic VesicleCopii-Coated Vesicle MembraneMembrane-Associated In The Presence Of Ca(2+)Localizes To Endoplasmic Reticulum Exit Site (Eres) |
| Alternative Antibody Names | Anti-Peflin antibodyAnti-Pef Protein With A Long N-Terminal Hydrophobic Domain antibodyAnti-Penta-Ef Hand Domain-Containing Protein 1 antibodyAnti-PEF1 antibodyAnti-ABP32 antibodyAnti-UNQ1845 antibodyAnti-PRO3573 antibody |
Information sourced from Uniprot.org