• Human appendix tissue was stained with Anti-CD31 (ABT-CD31) Antibody
  • Human Hemangioendothelioma tissue was stained with Anti-CD31 (ABT-CD31) Antibody
  • Human placenta tissue was stained with Anti-CD31 (ABT-CD31) Antibody
  • Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD31 (ABT-CD31) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: THP-1 Lane 2: HelaPredicted band size: 83kDaObserved band size: 130kDa
  • Various whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-CD31 (ABT-CD31) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Jurkat Lane 2: THP-1Predicted band size: 83kDaObserved band size: 130kDa
  • Immunohistochemical analysis of paraffin-embedded Hemangioendothelioma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Hemangioendothelioma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Placenta. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Placenta. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Placenta. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Liver. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Western blot analysis of CD31Antibody at 1:1000 dilution.

Anti-PECAM1 antibody (100-200 aa) [ABT-CD31] (STJ196788)

SKU:
STJ196788

£168.00 - £226.00
Current Stock:
Host: Mouse
Applications: WB/IHC/IF/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Platelet endothelial cell adhesion molecule (100-200 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Monoclonal
Clone ID: ABT-CD31
Conjugation: Unconjugated
Isotype: IgG1k
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: WB 1:200-1:500
IHC 1:20-50
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PECAM1
Gene ID: 5175
Uniprot ID: PECA1_HUMAN
Immunogen Region: 100-200 aa
Specificity: This antibody detects endogenous levels of human CD31. Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair method in paraffin section.
Immunogen: Synthesized peptide derived from the human CD31 at the amino acid range 100-200
Post Translational Modifications Phosphorylated on Ser and Tyr residues after cellular activation by src kinases. Upon activation, phosphorylated on Ser-729 which probably initiates the dissociation of the membrane-interaction segment (residues 709-729) from the cell membrane allowing the sequential phosphorylation of Tyr-713 and Tyr-690. Constitutively phosphorylated on Ser-734 in resting platelets. Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts.
Function Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells.PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes. Modulates bradykinin receptor BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Induces susceptibility to atherosclerosis. Isoform Delta15: Does not protect against apoptosis.
Protein Name Platelet Endothelial Cell Adhesion Molecule
Pecam-1
Endocam
Gpiia'
Peca1
Cd Antigen Cd31
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-202733
Reactome: R-HSA-210990
Reactome: R-HSA-216083
Reactome: R-HSA-432142
Reactome: R-HSA-6798695
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cell Surface Expression On Neutrophils Is Down-Regulated Upon Fmlp Or Cxcl8/Il8-Mediated Stimulation
Isoform Long: Cell Membrane
Membrane Raft
Cell Junction
Localizes To The Lateral Border Recycling Compartment (Lbrc) And Recycles From The Lbrc To The Junction In Resting Endothelial Cells
Isoform Delta15: Cell Junction
Alternative Antibody Names Anti-Platelet Endothelial Cell Adhesion Molecule antibody
Anti-Pecam-1 antibody
Anti-Endocam antibody
Anti-Gpiia' antibody
Anti-Peca1 antibody
Anti-Cd Antigen Cd31 antibody
Anti-PECAM1 antibody

Information sourced from Uniprot.org

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