Anti-PDX1 antibody [R03-6B9] (STJA0035783)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human PDX1
General Information
| Short Description | Rabbit monoclonal anti-PDX1 for use in WB, IHC-P, ICC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ICC/IF |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R03-6B9 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | PDX1 |
| Gene ID | 3651 |
| Uniprot ID | PDX1_HUMAN |
| Immunogen | A synthesized peptide derived from human PDX1 |
Additional Info
| Tissue Specificity | Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells). |
| Post Translational Modifications | Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration. Phosphorylated by HIPK2 on Ser-268 upon glucose accumulation. This phosphorylation mediates subnuclear localization shifting. Phosphorylation by PASK may lead to translocation into the cytosol. |
| Function | Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer.the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds preferentially the DNA motif 5'-CTTAATTG-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell. |
| Protein Name | Pancreas/Duodenum Homeobox Protein 1Pdx-1Glucose-Sensitive FactorGsfInsulin Promoter Factor 1Ipf-1Insulin Upstream Factor 1Iuf-1Islet/Duodenum Homeobox-1Idx-1Somatostatin-Transactivating Factor 1Stf-1 |
| Database Links | Reactome: R-HSA-210745Reactome: R-HSA-210747Reactome: R-HSA-9925561 |
| Cellular Localisation | NucleusCytoplasmCytosol |
| Alternative Antibody Names | Anti-Pancreas/Duodenum Homeobox Protein 1 antibodyAnti-Pdx-1 antibodyAnti-Glucose-Sensitive Factor antibodyAnti-Gsf antibodyAnti-Insulin Promoter Factor 1 antibodyAnti-Ipf-1 antibodyAnti-Insulin Upstream Factor 1 antibodyAnti-Iuf-1 antibodyAnti-Islet/Duodenum Homeobox-1 antibodyAnti-Idx-1 antibodyAnti-Somatostatin-Transactivating Factor 1 antibodyAnti-Stf-1 antibodyAnti-PDX1 antibodyAnti-IPF1 antibodyAnti-STF1 antibody |
Information sourced from Uniprot.org