• STJ70495 staining (0. 2µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Anti-PDLIM4/RIL antibody (C-Term) (STJ70495)

SKU:
STJ70495-100

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Host: Goat
Applications: Pep-ELISA/WB/IF
Reactivity: Human/Mouse/Rat/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-PDLIM4/RIL (C-Term) is suitable for use in ELISA, Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-Recommended for use at 0.1-0.5µg/ml
IHC-3.75µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: PDLIM4
Gene ID: 8572
Uniprot ID: PDLI4_HUMAN
Immunogen Region: C-Term
Accession Number: NP_003678.2
Immunogen Sequence: DVVAVYPNAKVELV
Post Translational Modifications Phosphorylated on tyrosine residue(s). Can be dephosphorylated by PTPN13.
Function Isoform 1: Suppresses SRC activation by recognizing and binding to active SRC and facilitating PTPN13-mediated dephosphorylation of SRC 'Tyr-419' leading to its inactivation. Inactivated SRC dissociates from this protein allowing the initiation of a new SRC inactivation cycle. Involved in reorganization of the actin cytoskeleton. In nonmuscle cells, binds to ACTN1 (alpha-actinin-1), increases the affinity of ACTN1 to F-actin (filamentous actin), and promotes formation of actin stress fibers. Involved in regulation of the synaptic AMPA receptor transport in dendritic spines of hippocampal pyramidal neurons directing the receptors toward an insertion at the postsynaptic membrane. Links endosomal surface-internalized GRIA1-containing AMPA receptors to the alpha-actinin/actin cytoskeleton. Increases AMPA receptor-mediated excitatory postsynaptic currents in neurons. Isoform 2: Involved in reorganization of the actin cytoskeleton and in regulation of cell migration. In response to oxidative stress, binds to NQO1, which stabilizes it and protects it from ubiquitin-independent degradation by the core 20S proteasome. Stabilized protein is able to heterodimerize with isoform 1 changing the subcellular location of it from cytoskeleton and nuclei to cytosol, leading to loss of isoforms 1 ability to induce formation of actin stress fibers. Counteracts the effects produced by isoform 1 on organization of actin cytoskeleton and cell motility to fine-tune actin cytoskeleton rearrangement and to attenuate cell migration.
Protein Name Pdz And Lim Domain Protein 4
Lim Protein Ril
Reversion-Induced Lim Protein
Cellular Localisation Isoform 1: Cytoplasm
Cytoskeleton
Nucleus
Cytoplasm
Perinuclear Region
Cell Projection
Lamellipodium
Dendritic Spine
Early Endosome Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Recycling Endosome Membrane
Synapse
Synaptosome
Localizes To Actin Stress Fibers In Nonmuscle Cells
Colocalizes With Gria1 In Early Endosomes
Enriched In Numerous But Not All Spine-Like Structures Along Dendritic Branches
Colocalizes With Actin And Enriched At Sites Containing Larger Amounts Of Actin And Alpha-Actinin
Targeted Efficiently To Spines Via Its Pdz Domain-Mediated Interaction With The Alpha-Actinin/Actin Cytoskeletal Complex
Localizes To Synaptosomes In Brain
Colocalizes With F-Actin
Colocalizes With Trip6 At Cell-Cell Contacts And Lamellipodia
In The Cytoplasm
Displays A Fibrillar Pattern With Characteristic Thick Fibers And Occasional Clusters
Colocalizes With The Actin Stress Fibers
Oxidative Stress Induces Redistribution From Cytoskeleton To Cytosol
Colocalizes With Src At The Perinuclear Region
But Not At Focal Adhesions
Isoform 2: Cytoplasm
Stains More Diffusely In The Cytoplasm With Thin Fibers Forming A Dense Mesh-Like Pattern
Alternative Antibody Names Anti-Pdz And Lim Domain Protein 4 antibody
Anti-Lim Protein Ril antibody
Anti-Reversion-Induced Lim Protein antibody
Anti-PDLIM4 antibody
Anti-RIL antibody

Information sourced from Uniprot.org

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