• Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain cells using 3 Mu g PDHX antibody (STJ28509). Western blot was performed from the immunoprecipitate using PDHX antibody (STJ28509) at a dilution of 1:1000.
  • Western blot analysis of various lysates using PDHX Rabbit polyclonal antibody (STJ28509) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-PDHX antibody (1-300) (STJ28509)

SKU:
STJ28509

£112.50 - £524.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IP:0.5 Mu g-4 Mu g antibody for 400 Mu g-600 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDHX
Gene ID: 8050
Uniprot ID: ODPX_HUMAN
Immunogen Region: 1-300
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PDHX (NP_003468.2).
Immunogen Sequence: MAASWRLGCDPRLLRYLVGF PGRRSVGLVKGALGWSVSRG ANWRWFHSTQWLRGDPIKIL MPSLSPTMEEGNIVKWLKKE GEAVSAGDALCEIETDKAVV TLDASDDGILAKIVVEEGSK NIRLGSLIGLIVEEGEDWKH VEIPKDVGPPPPVSKPSEPR PSPEPQISIPVKKEHIPGTL RFRLSPAARNILEKHSLDAS QGTATGPRGIFTKEDALKLV QLKQTGKITESRPTPAPTA
Post Translational Modifications Delipoylated at Lys-97 by SIRT4, delipoylation decreases the PHD complex activity.
Function Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex.
Protein Name Pyruvate Dehydrogenase Protein X Component - Mitochondrial
Dihydrolipoamide Dehydrogenase-Binding Protein Of Pyruvate Dehydrogenase Complex
E3-Binding Protein
E3bp
Lipoyl-Containing Pyruvate Dehydrogenase Complex Component X
Prox
Database Links Reactome: R-HSA-204174
Reactome: R-HSA-5362517
Reactome: R-HSA-9861559
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Pyruvate Dehydrogenase Protein X Component - Mitochondrial antibody
Anti-Dihydrolipoamide Dehydrogenase-Binding Protein Of Pyruvate Dehydrogenase Complex antibody
Anti-E3-Binding Protein antibody
Anti-E3bp antibody
Anti-Lipoyl-Containing Pyruvate Dehydrogenase Complex Component X antibody
Anti-Prox antibody
Anti-PDHX antibody
Anti-PDX1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance