• HEK293 overexpressing PDE5A (RC218974) and probed with STJ70656 (mock transfection in first lane) , tested by Origene.
  • STJ70656 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70656 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-PDE5A antibody (C-Term) (STJ70656)

SKU:
STJ70656-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB-Trf/IF/FC
Reactivity: Human/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-PDE5A (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1µg/ml
IF-Strong expression of the protein seen in the cytoplasm of HeLa cells. 10µg/ml
FC-Flow cytometric analysis of HEK293 cells. 10ug/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: PDE5A
Gene ID: 8654
Uniprot ID: PDE5A_HUMAN
Immunogen Region: C-Term
Accession Number: NP_001074.2; NP_246273.2; NP_236914.2; (NP_237223)
Specificity: This antibody is expected to recognise reported isoforms NP_001074.2, NP_236914.2 and NP_246273.2.
Immunogen Sequence: LINGESGQAKRN
Post Translational Modifications Phosphorylation is regulated by binding of cGMP to the two allosteric sites. Phosphorylation by PRKG1 leads to its activation.
Function Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. Specifically regulates nitric-oxide-generated cGMP.
Protein Name Cgmp-Specific 3' -5'-Cyclic Phosphodiesterase
Cgmp-Binding Cgmp-Specific Phosphodiesterase
Cgb-Pde
Database Links Reactome: R-HSA-418457
Reactome: R-HSA-445355
Reactome: R-HSA-9013422
Alternative Antibody Names Anti-Cgmp-Specific 3' -5'-Cyclic Phosphodiesterase antibody
Anti-Cgmp-Binding Cgmp-Specific Phosphodiesterase antibody
Anti-Cgb-Pde antibody
Anti-PDE5A antibody
Anti-PDE5 antibody

Information sourced from Uniprot.org

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