| Host: | Goat |
| Applications: | Pep-ELISA/WB-Trf/IF/FC |
| Reactivity: | Human/Rat/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-PDE5A (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:16000.WB: In transfected HEK293 transiently expressing PDE5A isoform 2 a band of approx. 95kDa is observed. This band is not observed in the non-transfected HEK293 (calculated MW of 94.8kDa acco |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | PDE5A |
| Gene ID: | 8654 |
| Uniprot ID: | PDE5A_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_001074.2; NP_246273.2; NP_236914.2; (NP_237223) |
| Specificity: | This antibody is expected to recognise reported isoforms NP_001074.2, NP_236914.2 and NP_246273.2. |
| Immunogen Sequence: | LINGESGQAKRN |
| Post Translational Modifications | Phosphorylation is regulated by binding of cGMP to the two allosteric sites. Phosphorylation by PRKG1 leads to its activation. |
| Function | Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. Specifically regulates nitric-oxide-generated cGMP. |
| Protein Name | Cgmp-Specific 3' -5'-Cyclic PhosphodiesteraseCgmp-Binding Cgmp-Specific PhosphodiesteraseCgb-Pde |
| Database Links | Reactome: R-HSA-418457Reactome: R-HSA-445355Reactome: R-HSA-9013422 |
| Alternative Antibody Names | Anti-Cgmp-Specific 3' -5'-Cyclic Phosphodiesterase antibodyAnti-Cgmp-Binding Cgmp-Specific Phosphodiesterase antibodyAnti-Cgb-Pde antibodyAnti-PDE5A antibodyAnti-PDE5 antibody |
Information sourced from Uniprot.org