| Host: |
Rabbit |
| Applications: |
CM/ELISA/ICC/IF/IHC/IP/WB |
| Reactivity: |
Chicken/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PDE4D is suitable for use in Confocal Microscopy, ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Biotin |
| Isotype: |
IgG |
| Purification: |
Affinity Purified |
| Concentration: |
0.68 µg/µl |
| Dilution Range: |
WB: 1:500DB: 1:10, 000ELISA: 1:10, 000IP: 1:150IHC: 1:200ICC: 1:200IF: 1:200CFM: 1:200 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
PDE4D |
| Gene ID: |
5144 |
| Uniprot ID: |
PDE4D_HUMAN |
| Specificity: |
Does not crossDoes not cross react with other PDE family members such as PDE4A, PDE4B or PDE4C. |
| Immunogen: |
Synthetic peptide corresponding to unique amino acid sequence on PDE4D3 variant. |
| Tissue Specificity | Expressed in colonic epithelial cells (at protein level). Widespread.most abundant in skeletal muscle. Isoform 6: Detected in brain. Isoform 8: Detected in brain, placenta, lung and kidney. Isoform 7: Detected in heart and skeletal muscle. |
| Post Translational Modifications | Long isoforms that share a conserved PKA phosphorylation site in the N-terminus are activated by PKA through phosphorylation. Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59. Sumoylation of long isoforms by PIAS4 augments their activation by PKA phosphorylation and represses their inhibition by ERK phosphorylation. |
| Function | Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. |
| Protein Name | Camp-Specific 3' -5'-Cyclic Phosphodiesterase 4dDpde3Pde43 |
| Database Links | Reactome: R-HSA-180024Reactome: R-HSA-418555 |
| Cellular Localisation | Apical Cell MembraneCytoplasmMembraneCytoskeletonMicrotubule Organizing CenterCentrosomeFound In The Soluble FractionAssociated With MembranesAnd Associated With The Cytoskeleton And The CentrosomeColocalized With Shank2 To The Apical Membrane Of Colonic Crypt Cells |
| Alternative Antibody Names | Anti-Camp-Specific 3' -5'-Cyclic Phosphodiesterase 4d antibodyAnti-Dpde3 antibodyAnti-Pde43 antibodyAnti-PDE4D antibodyAnti-DPDE3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
