• Immunofluorescence analysis of NIH/3T3 cells using PDE12 Rabbit polyclonal antibody (STJ11102898) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using PDE12 Rabbit polyclonal antibody (STJ11102898) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using PDE12 Rabbit polyclonal antibody (STJ11102898) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PDE12 antibody (17-300) (STJ11102898)

SKU:
STJ11102898

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PDE12 (17-300) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDE12
Gene ID: 201626
Uniprot ID: PDE12_HUMAN
Immunogen Region: 17-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 17-300 of human PDE12 (NP_001309106.1).
Immunogen Sequence: AVEKLSRAEAGSQTAAGAME RAVVRCVPSEPKLSLSFALA DGSHKNMQRDQSEPLGRVLS RIATNALKGHAKAAAAKKSR KSRPNASGGAACSGPGPEPA VFCEPVVKLYYREEAVAEDV LNVDAWQDGAVLQIGDVKYK VERNPPAFTELQLPRYIMAG FPVCPKLSLEFGDPASSLFR WYKEAKPGAAEPEVGVPSSL SPSSPSSSWTETDVEERVYT PSNADIGLRLKLHCTPGDG
Tissue Specificity Ubiquitous.
Function Enzyme that cleaves 2',5'-phosphodiester bond linking adenosines of the 5'-triphosphorylated oligoadenylates, triphosphorylated oligoadenylates referred as 2-5A modulates the 2-5A system. Degrades triphosphorylated 2-5A to produce AMP and ATP. Also cleaves 3',5'-phosphodiester bond of oligoadenylates. Plays a role as a negative regulator of the 2-5A system that is one of the major pathways for antiviral and antitumor functions induced by interferons (IFNs). Suppression of this enzyme increases cellular 2-5A levels and decreases viral replication in cultured small-airway epithelial cells and Hela cells.
Protein Name 2' -5'-Phosphodiesterase 12
2'-Pde
2-Pde
Mitochondrial Deadenylase
Database Links Reactome: R-HSA-8983711
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-2' -5'-Phosphodiesterase 12 antibody
Anti-2'-Pde antibody
Anti-2-Pde antibody
Anti-Mitochondrial Deadenylase antibody
Anti-PDE12 antibody

Information sourced from Uniprot.org

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