| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PCNT (95-212) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PCNT |
| Gene ID: |
5116 |
| Uniprot ID: |
PCNT_HUMAN |
| Immunogen Region: |
95-212 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 95-212 of human PCNT (NP_006022.3). |
| Immunogen Sequence: |
GEKREDLEQLQQKQVNDHPP EQCGMFTVSDHPPEQHGMFT VGDHPPEQRGMFTVSDHPPE QHGMFTVSDHPPEQRGMFTI SDHQPEQRGMFTVSDHTPEQ RGIFTISDHPAEQRGMFT |
| Tissue Specificity | Expressed in all tissues tested, including placenta, liver, kidney and thymus. |
| Post Translational Modifications | Cleaved during mitotis which leads to removal of CDK5RAP2 from the centrosome and promotes centriole disengagement and subsequent centriole separation. The C-terminal fragment is rapidly degraded following cleavage. Ubiquitinated by TRIM43.leading to proteasomal degradation. |
| Function | Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome. |
| Protein Name | PericentrinKendrinPericentrin-B |
| Database Links | Reactome: R-HSA-2565942Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-5620912Reactome: R-HSA-8854518Reactome: R-HSA-9613829Reactome: R-HSA-9615710Reactome: R-HSA-9646399 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentrosomal At All Stages Of The Cell CycleRemains Associated With Centrosomes Following Microtubule DepolymerizationColocalized With Disc1 At The Centrosome |
| Alternative Antibody Names | Anti-Pericentrin antibodyAnti-Kendrin antibodyAnti-Pericentrin-B antibodyAnti-PCNT antibodyAnti-KIAA0402 antibodyAnti-PCNT2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and PDCD10 knockout (KO) HeLa cells, using [KO Validated] PDCD10 Rabbit polyclonal antibody (STJ117595) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/96554/379107/STJ117595_1__37100.1713146827.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PDCD10 knockout (KO) HeLa cells, using [KO Validated] PDCD10 Rabbit polyclonal antibody (STJ117595) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.")
