• Western blot analysis of extracts of various cell lines, using PEPCK/PEPCK/PCK2 antibody (STJ110744) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% non-fat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 10s.
  • Immunohistochemistry of paraffin-embedded mouse kidney using PEPCK/PEPCK/PCK2 rabbit polyclonal antibody (STJ110744) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat kidney using PEPCK/PEPCK/PCK2 rabbit polyclonal antibody (STJ110744) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of NIH-3T3 cells using PEPCK/PEPCK/PCK2 rabbit polyclonal antibody (STJ110744) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using PEPCK/PEPCK/PCK2 rabbit polyclonal antibody (STJ110744) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of extracts of HepG2 cells using PEPCK/PEPCK/PCK2 antibody (STJ110744). Western blot was performed from the immunoprecipitate using PEPCK/PEPCK/PCK2 antibody (STJ110744) at a dilition of 1:1000.

Anti-PCK2 antibody (1-280) (STJ110744)

SKU:
STJ110744

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PCK2 (1-280) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PCK2
Gene ID: 5106
Uniprot ID: PCKGM_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PEPCK/PEPCK/PCK2 (NP_004554.2).
Immunogen Sequence: MAALYRPGLRLNWHGLSPLG WPSCRSIQTLRVLSGDLGQL PTGIRDFVEHSARLCQPEGI HICDGTEAENTATLTLLEQQ GLIRKLPKYNNCWLARTDPK DVARVESKTVIVTPSQRDTV QLPPGGARGQLGNWMSPADF QRAVDERFPGCMQGRTMYVL PFSMGPVGSPLSRIGVQLTD SAYVVASMRIMTRLGTPVLQ ALGDGDFVKCLHSVGQPLTG QGEPVSQWPCNPEKTLIGH
Function Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
Protein Name Phosphoenolpyruvate Carboxykinase Gtp - Mitochondrial
Pepck-M
Database Links Reactome: R-HSA-70263
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Phosphoenolpyruvate Carboxykinase Gtp - Mitochondrial antibody
Anti-Pepck-M antibody
Anti-PCK2 antibody
Anti-PEPCK2 antibody

Information sourced from Uniprot.org

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