Anti-PCIF1 antibody (1-200) (STJ11104434)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Mrna (1-200) antibody for use in WB, IHC-P, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:100IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PCIF1 |
| Gene ID | 63935 |
| Uniprot ID | CAPAM_HUMAN |
| Immunogen Region | 1-200 |
| Immunogen Sequence | MANENHGSPREEASLLSHSP GTSNQSQPCSPKPIRLVQDL PEELVHAGWEKCWSRRENRP YYFNRFTNQSLWEMPVLGQH DVISDPLGLNATPLPQDSSL VETPPAENKPRKRQLSEEQP SGNGVKKPKIEIPVTPTGQS VPSSPSIPGTPTLKMWGTSP EDKQQAALLRPTEVYWDLDI QTNAVIKHRGPSEVLPPHPE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PCIF1 (NP_071387.1). |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Function | Cap-specific adenosine methyltransferase that catalyzes formation of N(6),2'-O-dimethyladenosine cap (m6A(m)) by methylating the adenosine at the second transcribed position of capped mRNAs. Recruited to the early elongation complex of RNA polymerase II (RNAPII) via interaction with POLR2A and mediates formation of m6A(m) co-transcriptionally. |
| Protein Name | Mrna2'-O-Methyladenosine-N(6--MethyltransferaseCap-Specific Adenosine MethyltransferaseCapamHcapamPhosphorylated Ctd-Interacting Factor 1Hpcif1Protein Phosphatase 1 Regulatory Subunit 121 |
| Database Links | |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Mrna antibodyAnti-2'-O-Methyladenosine-N(6--Methyltransferase antibodyAnti-Cap-Specific Adenosine Methyltransferase antibodyAnti-Capam antibodyAnti-Hcapam antibodyAnti-Phosphorylated Ctd-Interacting Factor 1 antibodyAnti-Hpcif1 antibodyAnti-Protein Phosphatase 1 Regulatory Subunit 121 antibodyAnti-PCIF1 antibodyAnti-C20orf67 antibodyAnti-CAPAM antibodyAnti-PPP1R121 antibody |
Information sourced from Uniprot.org