• Western blot analysis of various lysates using PCF11 Rabbit polyclonal antibody (STJ118960) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PCF11 antibody (1256-1555) (STJ118960)

SKU:
STJ118960

€140.63 - €653.13
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PCF11 (1256-1555) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PCF11
Gene ID: 51585
Uniprot ID: PCF11_HUMAN
Immunogen Region: 1256-1555
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1256-1555 of human PCF11 (NP_056969.2).
Immunogen Sequence: PKAYPDNHLSQVDVNELFSK LLKTGILKLSQTDSATTQVS EVTAQPPPEEEEDQNEDQDV PDLTNFTVEELKQRYDSVIN RLYTGIQCYSCGMRFTTSQT DVYADHLDWHYRQNRTEKDV SRKVTHRRWYYSLTDWIEFE EIADLEERAKSQFFEKVHEE VVLKTQEAAKEKEFQSVPAG PAGAVESCEICQEQFEQYWD EEEEEWHLKNAIRVDGKIYH PSCYEDYQNTSSFDCTPSP
Post Translational Modifications Phosphorylation at Ser-120 and/or Thr-121 by WNK1 weakens its association with POLR2A/RNA polymerase II, promoting transcript release from the chromatin template and mRNA export to the cytoplasm.
Function Component of pre-mRNA cleavage complex II, which promotes transcription termination by RNA polymerase II.
Protein Name Pre-Mrna Cleavage Complex 2 Protein Pcf11
Pre-Mrna Cleavage Complex Ii Protein Pcf11
Database Links Reactome: R-HSA-6807505
Reactome: R-HSA-72187
Reactome: R-HSA-72203
Reactome: R-HSA-73856
Reactome: R-HSA-77595
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Pre-Mrna Cleavage Complex 2 Protein Pcf11 antibody
Anti-Pre-Mrna Cleavage Complex Ii Protein Pcf11 antibody
Anti-PCF11 antibody
Anti-KIAA0824 antibody

Information sourced from Uniprot.org

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