| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PCBP2 |
| Gene ID: | 5094 |
| Uniprot ID: | PCBP2_HUMAN |
| Immunogen Region: | 199-215 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 150-250 of human hnRNP E2/PCBP2 (NP_001122383.1). |
| Immunogen Sequence: | GIPQSIIECVKQICVVMLET LSQSPPKGVTIPYRPKPSSS PVIFAGGQDRYSTGSDSASF PHTTPSMCLNPDLEGPPLEA YTIQGQYAIPQPDLTKLHQL AMQQSHFPMTHGNTGFSGIE SSSPEVKGYWGLDASAQTTS HELTIPNDLIGCIIGRQGAK INEIRQMSGAQIKIANPVEG STDRQVTITGSAASISLAQY LINVRLSSETGGMGSS |
| Tissue Specificity | Detected in all tissues examined. |
| Post Translational Modifications | Phosphorylated. The non-phosphorylated form(s) exhibited the strongest poly(rC)-binding activity. (Microbial infection) Proteolytically cleaved by picornavirus proteinase 3CD. |
| Function | Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Acts as a negative regulator of antiviral signaling. Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitination and degradation. Negativeley regulates the cGAS-STING pathway via interaction with CGAS, preventing the formation of liquid-like droplets in which CGAS is activated. Together with PCBP1, required for erythropoiesis, possibly by regulating mRNA splicing. (Microbial infection) In case of infection by poliovirus, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation. Also plays a role in initiation of viral RNA replication in concert with the viral protein 3CD. |
| Protein Name | Poly(Rc-Binding Protein 2 Alpha-Cp2 Heterogeneous Nuclear Ribonucleoprotein E2 Hnrnp E2 |
| Database Links | Reactome: R-HSA-72163 Reactome: R-HSA-72203 Reactome: R-HSA-936440 Reactome: R-HSA-9692916 |
| Cellular Localisation | Nucleus Cytoplasm Loosely Bound In The Nucleus May Shuttle Between The Nucleus And The Cytoplasm |
| Alternative Antibody Names | Anti-Poly(Rc-Binding Protein 2 antibody Anti-Alpha-Cp2 antibody Anti-Heterogeneous Nuclear Ribonucleoprotein E2 antibody Anti-Hnrnp E2 antibody Anti-PCBP2 antibody |
Information sourced from Uniprot.org