• Western blot analysis of extracts of various cell lines, using hnRNP E2/PCBP2 rabbit polyclonal antibody antibody (STJ24914) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using hnRNP E2/PCBP2 rabbit polyclonal antibody (STJ24914) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using hnRNP E2/PCBP2 rabbit polyclonal antibody (STJ24914) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH-3T3 cells using hnRNP E2/PCBP2 rabbit polyclonal antibody (STJ24914) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using hnRNP E2/PCBP2 rabbit polyclonal antibody (STJ24914) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using hnRNP E2/PCBP2 rabbit polyclonal antibody (STJ24914) at dilution of 1:400 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells using hnRNP E2/PCBP2 rabbit polyclonal antibody (STJ24914) at dilution of 1:400 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using hnRNP E2/PCBP2 rabbit polyclonal antibody (STJ24914) at dilution of 1:400 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using hnRNP E2/PCBP2 rabbit polyclonal antibody (STJ24914) at dilution of 1:400 (40x lens). Blue: DAPI for nuclear staining.

Anti-PCBP2 antibody (199-215) (STJ24914)

SKU:
STJ24914

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PCBP2 (199-215) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PCBP2
Gene ID: 5094
Uniprot ID: PCBP2_HUMAN
Immunogen Region: 199-215
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 199-215 of human hnRNP E2/PCBP2 (NP_001122383.1).
Immunogen Sequence: RYSTGSDSASFPHTTPS
Tissue Specificity Detected in all tissues examined.
Post Translational Modifications Phosphorylated. The non-phosphorylated form(s) exhibited the strongest poly(rC)-binding activity. (Microbial infection) Proteolytically cleaved by picornavirus proteinase 3CD.
Function Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Acts as a negative regulator of antiviral signaling. Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitination and degradation. Negativeley regulates the cGAS-STING pathway via interaction with CGAS, preventing the formation of liquid-like droplets in which CGAS is activated. Together with PCBP1, required for erythropoiesis, possibly by regulating mRNA splicing. (Microbial infection) In case of infection by poliovirus, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation. Also plays a role in initiation of viral RNA replication in concert with the viral protein 3CD.
Protein Name Poly(Rc-Binding Protein 2
Alpha-Cp2
Heterogeneous Nuclear Ribonucleoprotein E2
Hnrnp E2
Database Links Reactome: R-HSA-72163
Reactome: R-HSA-72203
Reactome: R-HSA-936440
Reactome: R-HSA-9692916
Cellular Localisation Nucleus
Cytoplasm
Loosely Bound In The Nucleus
May Shuttle Between The Nucleus And The Cytoplasm
Alternative Antibody Names Anti-Poly(Rc-Binding Protein 2 antibody
Anti-Alpha-Cp2 antibody
Anti-Heterogeneous Nuclear Ribonucleoprotein E2 antibody
Anti-Hnrnp E2 antibody
Anti-PCBP2 antibody

Information sourced from Uniprot.org

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