| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Dog |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-PBX1 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000. WB: Approx 45kDa band observed in lysates of cell line K562, in Mouse and Rat Eye and in Rat Uterus lysates (calculated MW of 46.6kDa according to Human NP_002576.1). Recommended concentra |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | PBX1 |
| Gene ID: | 5087 |
| Uniprot ID: | PBX1_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_002576.1 |
| Immunogen Sequence: | ATNVSAHGSQANSPS |
| Function | Transcription factor which binds the DNA sequence 5'-TGATTGAT-3' as part of a heterodimer with HOX proteins such as HOXA1, HOXA5, HOXB7 and HOXB8. Binds to the DNA sequence 5'-TGATTGAC-3' in complex with a nuclear factor which is not a class I HOX protein. Has also been shown to bind the DNA sequence 5'-ATCAATCAA-3' cooperatively with HOXA5, HOXB7, HOXB8, HOXC8 and HOXD4. Acts as a transcriptional activator of PF4 in complex with MEIS1. Also activates transcription of SOX3 in complex with MEIS1 by binding to the 5'-TGATTGAC-3' consensus sequence. In natural killer cells, binds to the NFIL3 promoter and acts as a transcriptional activator of NFIL3, promoting natural killer cell development. Plays a role in the cAMP-dependent regulation of CYP17A1 gene expression via its cAMP-regulatory sequence (CRS1). Probably in complex with MEIS2, involved in transcriptional regulation by KLF4. Acts as a transcriptional activator of NKX2-5 and a transcriptional repressor of CDKN2B. Together with NKX2-5, required for spleen development through a mechanism that involves CDKN2B repression. Isoform PBX1b: As part of a PDX1:PBX1b:MEIS2B complex in pancreatic acinar cells, is involved in the transcriptional activation of the ELA1 enhancer.the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. |
| Protein Name | Pre-B-Cell Leukemia Transcription Factor 1 Homeobox Protein Pbx1 Homeobox Protein Prl |
| Database Links | Reactome: R-HSA-452723 Reactome: R-HSA-5617472 Reactome: R-HSA-9013508 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Pre-B-Cell Leukemia Transcription Factor 1 antibody Anti-Homeobox Protein Pbx1 antibody Anti-Homeobox Protein Prl antibody Anti-PBX1 antibody Anti-PRL antibody |
Information sourced from Uniprot.org