Anti-PBX1 antibody (311-430) (STJ29704)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PBX1 (311-430) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PBX1 |
| Gene ID | 5087 |
| Uniprot ID | PBX1_HUMAN |
| Immunogen Region | 311-430 |
| Immunogen Sequence | VTATNVSAHGSQANSPSTPN SAGSSSSFNMSNSGDLFMSV QSLNGDSYQGAQVGANVQSQ VDTLRHVISQTGGYSDGLAA SQMYSPQGISANGGWQDATT PSSVTSPTEGPGSVHSDTSN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 311-430 of human PBX1 (NP_002576.1). |
Additional Info
| Tissue Specificity | Expressed in the kidney. Expressed in the endothelial cells of the glomeruli and interstitium (at protein level). Expressed in all tissues except in cells of the B and T lineage. Expressed strongly in kidney and brain. |
| Function | Transcription factor which binds the DNA sequence 5'-TGATTGAT-3' as part of a heterodimer with HOX proteins such as HOXA1, HOXA5, HOXB7 and HOXB8. Binds to the DNA sequence 5'-TGATTGAC-3' in complex with a nuclear factor which is not a class I HOX protein. Has also been shown to bind the DNA sequence 5'-ATCAATCAA-3' cooperatively with HOXA5, HOXB7, HOXB8, HOXC8 and HOXD4. Acts as a transcriptional activator of PF4 in complex with MEIS1. Also activates transcription of SOX3 in complex with MEIS1 by binding to the 5'-TGATTGAC-3' consensus sequence. In natural killer cells, binds to the NFIL3 promoter and acts as a transcriptional activator of NFIL3, promoting natural killer cell development. Plays a role in the cAMP-dependent regulation of CYP17A1 gene expression via its cAMP-regulatory sequence (CRS1). Probably in complex with MEIS2, involved in transcriptional regulation by KLF4. Acts as a transcriptional activator of NKX2-5 and a transcriptional repressor of CDKN2B. Together with NKX2-5, required for spleen development through a mechanism that involves CDKN2B repression. Isoform PBX1b: As part of a PDX1:PBX1b:MEIS2B complex in pancreatic acinar cells, is involved in the transcriptional activation of the ELA1 enhancer.the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. |
| Protein Name | Pre-B-Cell Leukemia Transcription Factor 1Homeobox Protein Pbx1Homeobox Protein Prl |
| Database Links | Reactome: R-HSA-452723Reactome: R-HSA-5617472Reactome: R-HSA-9013508 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Pre-B-Cell Leukemia Transcription Factor 1 antibodyAnti-Homeobox Protein Pbx1 antibodyAnti-Homeobox Protein Prl antibodyAnti-PBX1 antibodyAnti-PRL antibody |
Information sourced from Uniprot.org