• Immunofluorescence analysis of MCF-7 cells using PAX3 Rabbit polyclonal antibody (STJ24909). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of PAX3 in paraffin-embedded human colon carcinoma using PAX3 Rabbit polyclonal antibody (STJ24909) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using PAX3 Rabbit polyclonal antibody (STJ24909) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PAX3 antibody (1-479) (STJ24909)

SKU:
STJ24909

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PAX3 (1-479) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PAX3
Gene ID: 5077
Uniprot ID: PAX3_HUMAN
Immunogen Region: 1-479
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-479 of human PAX3 (NP_852122.1).
Immunogen Sequence: MTTLAGAVPRMMRPGPGQNY PRSGFPLEVSTPLGQGRVNQ LGGVFINGRPLPNHIRHKIV EMAHHGIRPCVISRQLRVSH GCVSKILCRYQETGSIRPGA IGGSKPKQVTTPDVEKKIEE YKRENPGMFSWEIRDKLLKD AVCDRNTVPSVSSISRILRS KFGKGEEEEADLERKEAEES EKKAKHSIDGILSERASAPQ SDEGSDIDSEPDLPLKRKQR RSRTTFTAEQLEELER
Function Transcription factor that may regulate cell proliferation, migration and apoptosis. Involved in neural development and myogenesis. Transcriptional activator of MITF, acting synergistically with SOX10.
Protein Name Paired Box Protein Pax-3
Hup2
Database Links Reactome: R-HSA-3214847
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Paired Box Protein Pax-3 antibody
Anti-Hup2 antibody
Anti-PAX3 antibody
Anti-HUP2 antibody

Information sourced from Uniprot.org

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