• Western blot analysis of extracts of various cell lines, using PARP4 antibody (STJ110641) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of extracts of various cell lines, using PARP4 antibody (STJ110641) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-PARP4 antibody (1-300) (STJ110641)

SKU:
STJ110641

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PARP4 (1-300) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PARP4
Gene ID: 143
Uniprot ID: PARP4_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PARP4 (NP_006428.2).
Immunogen Sequence: MVMGIFANCIFCLKVKYLPQ QQKKKLQTDIKENGGKFSFS LNPQCTHIILDNADVLSQYQ LNSIQKNHVHIANPDFIWKS IREKRLLDVKNYDPYKPLDI TPPPDQKASSSEVKTEGLCP DSATEEEDTVELTEFGMQNV EIPHLPQDFEVAKYNTLEKV GMEGGQEAVVVELQCSRDSR DCPFLISSHFLLDDGMETRR QFAIKKTSEDASEYFENYIE ELKKQGFLLREHFTPEATQ
Tissue Specificity Widely expressed.the highest levels are in the kidney.also detected in heart, placenta, lung, liver, skeletal muscle, spleen, leukocytes and pancreas.
Function Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins.
Protein Name Protein Mono-Adp-Ribosyltransferase Parp4
193 Kda Vault Protein
Adp-Ribosyltransferase Diphtheria Toxin-Like 4
Artd4
Parp-Related/Ialphai-Related H5/Proline-Rich
Ph5p
Poly Adp-Ribose Polymerase 4
Parp-4
Vault Poly(Adp-Ribose Polymerase
Vparp
Database Links Reactome: R-HSA-197264
Reactome: R-HSA-9683610
Reactome: R-HSA-9694631
Cellular Localisation Cytoplasm
Nucleus
Cytoskeleton
Spindle
Also Found In The Nucleus
Associated With Mitotic Spindles
Alternative Antibody Names Anti-Protein Mono-Adp-Ribosyltransferase Parp4 antibody
Anti-193 Kda Vault Protein antibody
Anti-Adp-Ribosyltransferase Diphtheria Toxin-Like 4 antibody
Anti-Artd4 antibody
Anti-Parp-Related/Ialphai-Related H5/Proline-Rich antibody
Anti-Ph5p antibody
Anti-Poly Adp-Ribose Polymerase 4 antibody
Anti-Parp-4 antibody
Anti-Vault Poly(Adp-Ribose Polymerase antibody
Anti-Vparp antibody
Anti-PARP4 antibody
Anti-ADPRTL1 antibody
Anti-KIAA0177 antibody
Anti-PARPL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance