Anti-PARP10 antibody (1-300) (STJ114681)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PARP10 (1-300) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PARP10 |
| Gene ID | 84875 |
| Uniprot ID | PAR10_HUMAN |
| Immunogen Region | 1-300 |
| Immunogen Sequence | MVAMAEAEAGVAVEVRGLPP AVPDELLTLYFENRRRSGGG PVLSWQRLGCGGVLTFREPA DAERVLAQADHELHGAQLSL RPAPPRAPARLLLQGLPPGT TPQRLEQHVQALLRASGLPV QPCCALASPRPDRALVQLPK PLSEADVRVLEEQAQNLGLE GTLVSLARVPQARAVRVVGD GASVDLLLLELYLENERRSG GGPLEDLQRLPGPLGTVASF QQWQVAERVLQQEHRLQGS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PARP10 (NP_116178.2). |
Additional Info
| Tissue Specificity | Highly expressed in spleen and thymus. Intermediate levels in liver, kidney, pancreas, prostate, testis, ovary, intestine, and leukocytes. Low expression in heart, brain, placenta, lung, skeletal muscle, and colon. |
| Post Translational Modifications | Stimulated through its phosphorylation by CDK2. Acquires CDK-dependent phosphorylation through late-G1 to S phase, and from prometaphase to cytokinesis in the nucleolar organizing regions. Phosphorylation is suppressed in growth-arrested cells. Auto-mono-ADP-ribosylated on glutamate and lysine residues. |
| Function | ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate and aspartate residues on target proteins. In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation. Catalyzes mono-ADP-ribosylation of GSK3B, leading to negatively regulate GSK3B kinase activity. Involved in translesion DNA synthesis in response to DNA damage via its interaction with PCNA. |
| Protein Name | Protein Mono-Adp-Ribosyltransferase Parp10Adp-Ribosyltransferase Diphtheria Toxin-Like 10Artd10Poly Adp-Ribose Polymerase 10Parp-10 |
| Database Links | Reactome: R-HSA-197264Reactome: R-HSA-9683610Reactome: R-HSA-9694631 |
| Cellular Localisation | NucleusNucleolusCytoplasmShuttles Between The Nuclear And Cytoplasmic CompartmentA Subpopulation Concentrates In The Nucleolus During Late G1/S Phase |
| Alternative Antibody Names | Anti-Protein Mono-Adp-Ribosyltransferase Parp10 antibodyAnti-Adp-Ribosyltransferase Diphtheria Toxin-Like 10 antibodyAnti-Artd10 antibodyAnti-Poly Adp-Ribose Polymerase 10 antibodyAnti-Parp-10 antibodyAnti-PARP10 antibody |
Information sourced from Uniprot.org