• Western blot analysis of various lysates using Pan-Succinyl-K Rabbit polyclonal antibody (STJ11103353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Pan-Succinyl-K antibody (STJ11103353)

SKU:
STJ11103353

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Specificity: A synthetic peptide corresponding to a sequence containing Succinyl-K.
Background Succinylation of protein is a kind of post-translational modification of protein mainly occurring on lysine residues. Compared with methylation and acetylation, succinylation of lysine has a greater effect on protein structure and function. Sirt5 is an important de-succinylation modifying enzyme involved in the regulation of a variety of biological processes, such as reactive oxygen species defense, fatty acid metabolism and cell apoptosis

Information sourced from Uniprot.org

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