• Hela whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Caldesmon (ABT125) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 93kDaObserved band size: 93kDa
  • Whole cell lysates of Hela were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Caldesmon antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Predicted band size: 93 (75) kDa
  • Human appendix tissue was stained with anti-Caldesmon (ABT125) antibody.
  • Human smooth muscle tissue was stained with anti-Caldesmon (ABT125) antibody.
  • Fluorescence multiplex immunohistochemical analysis of normal human appendix tissue (formalin-fixed paraffin-embedded section). The section was incubated in 3 rounds of staining; in the order of CK PAN. (STJ197095 1/200 dilution), PD-1. (STJ196857 1/200 dilution), Caldesmon pan. (STJ197106 1/200 dilution), each using a separate fluorescent tyramide signal amplification system : Treble-Fluorescence immunohistochemical mouse/rabbit kit (pH9.0)

Anti-PAN-CALD1 antibody (100-200 aa) [ABT125] (STJ197106)

SKU:
STJ197106

£168.00 - £226.00
Current Stock:
Host: Mouse
Applications: IHC/WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-PAN-Caldesmon (100-200 aa) is suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: ABT125
Conjugation: Unconjugated
Isotype: IgG2ak
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: IHC-P 1:100-500
WB 1:200-1000
IF 1:100-500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CALD1
Gene ID: 800
Uniprot ID: CALD1_HUMAN
Immunogen Region: 100-200 aa
Specificity: The antibody can recognize human h-caldesmon and l-caldesmon protein. In western blotting, the antibody labels a 93KDa band.
Immunogen: Synthesized peptide derived from the human Caldesmon pan at the amino acid range 100-200
Post Translational Modifications In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization. CDK1-mediated phosphorylation promotes Schwann cell migration during peripheral nerve regeneration.
Function Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration.
Protein Name Caldesmon
Cdm
Database Links Reactome: R-HSA-445355
Cellular Localisation Cytoplasm
Cytoskeleton
Myofibril
Stress Fiber
On Thin Filaments In Smooth Muscle And On Stress Fibers In Fibroblasts (Nonmuscle)
Alternative Antibody Names Anti-Caldesmon antibody
Anti-Cdm antibody
Anti-CALD1 antibody
Anti-CAD antibody
Anti-CDM antibody

Information sourced from Uniprot.org

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