Western blot analysis of various lysates using PAM16 Rabbit pAb (STJ111917) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-PAM16 antibody (21-125) (STJ111917)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ111917
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General Information

Short DescriptionRabbit polyclonal PAM16 (21-125) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolPAM16
Gene ID51025
Uniprot IDTIM16_HUMAN
Immunogen Region21-125
Immunogen SequenceARALRQEFAASRAAADARGR AGHRSAAASNLSGLSLQEAQ QILNVSKLSPEEVQKNYEHL FKVNDKSVGGSFYLQSKVVR AKERLDEELKIQAQEDREKG QMPHT
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 21-125 of human PAM16 (NP_057153.8).

Additional Info

Tissue Specificity Ubiquitously expressed.
Function Regulates ATP-dependent protein translocation into the mitochondrial matrix. Inhibits DNAJC19 stimulation of HSPA9/Mortalin ATPase activity.
Protein Name Mitochondrial Import Inner Membrane Translocase Subunit Tim16
Mitochondria-Associated Granulocyte Macrophage Csf-Signaling Molecule
Presequence Translocated-Associated Motor Subunit Pam16
Database Links Reactome: R-HSA-1268020
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Mitochondrial Import Inner Membrane Translocase Subunit Tim16 antibody
Anti-Mitochondria-Associated Granulocyte Macrophage Csf-Signaling Molecule antibody
Anti-Presequence Translocated-Associated Motor Subunit Pam16 antibody
Anti-PAM16 antibody
Anti-MAGMAS antibody
Anti-TIM16 antibody
Anti-TIMM16 antibody
Anti-CGI-136 antibody

Information sourced from Uniprot.org

Citations

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