• Immunofluorescence analysis of L929 cells using PALD1 Rabbit polyclonal antibody (STJ114759) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using PALD1 Rabbit polyclonal antibody (STJ114759) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PALD1 antibody (1-300) (STJ114759)

SKU:
STJ114759

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PALD1 (1-300) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PALD1
Gene ID: 27143
Uniprot ID: PALD_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PALD1 (NP_055246.2).
Immunogen Sequence: MGTTASTAQQTVSAGTPFEG LQGSGTMDSRHSVSIHSFQS TSLHNSKAKSIIPNKVAPVV ITYNCKEEFQIHDELLKAHY TLGRLSDNTPEHYLVQGRYF LVRDVTEKMDVLGTVGSCGA PNFRQVQGGLTVFGMGQPSL SGFRRVLQKLQKDGHRECVI FCVREEPVLFLRADEDFVSY TPRDKQNLHENLQGLGPGVR VESLELAIRKEIHDFAQLSE NTYHVYHNTEDLWGEPHAV
Tissue Specificity Expressed in endothelial cells, and in certain larger vessels, in mural cells. In the brain, possibly expressed in microglia. Expressed in peripheral blood mononuclear cells (at protein level).
Protein Name Paladin
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Paladin antibody
Anti-PALD1 antibody
Anti-KIAA1274 antibody
Anti-PALD antibody

Information sourced from Uniprot.org

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