• Western blot analysis of extracts of LNCaP cells, using PAK4 antibody (STJ113249) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of extracts of various cell lines, using PAK4 antibody (STJ113249) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of NIH/3T3 cells using PAK4 rabbit polyclonal antibody (STJ113249) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-PAK4 antibody (450-550) (STJ113249)

SKU:
STJ113249

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PAK4 (450-550) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PAK4
Gene ID: 10298
Uniprot ID: PAK4_HUMAN
Immunogen Region: 450-550
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 450-550 of human PAK4 (NP_005875.1).
Immunogen Sequence: HDGRVKLSDFGFCAQVSKEV PRRKSLVGTPYWMAPELISR LPYGPEVDIWSLGIMVIEMV DGEPPYFNEPPLKAMKMIRD NLPPRLKNLHKVSPSLKGFL D
Tissue Specificity Highest expression in prostate, testis and colon.
Post Translational Modifications Autophosphorylated on serine residues when activated by CDC42/p21 (Ref.32). Phosphorylated on tyrosine residues upon stimulation of FGFR2. Methylated by SETD6. Polyubiquitinated, leading to its proteasomal degradation.
Function Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.
Protein Name Serine/Threonine-Protein Kinase Pak 4
P21-Activated Kinase 4
Pak-4
Database Links Reactome: R-HSA-428540
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013406
Reactome: R-HSA-9013407
Reactome: R-HSA-9013408
Reactome: R-HSA-9013409
Reactome: R-HSA-9013420
Reactome: R-HSA-9013423
Reactome: R-HSA-9013424
Cellular Localisation Cytoplasm
Seems To Shuttle Between Cytoplasmic Compartments Depending On The Activating Effector
For Example
Can Be Found On The Cell Periphery After Activation Of Growth-Factor Or Integrin-Mediated Signaling Pathways
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Pak 4 antibody
Anti-P21-Activated Kinase 4 antibody
Anti-Pak-4 antibody
Anti-PAK4 antibody
Anti-KIAA1142 antibody

Information sourced from Uniprot.org

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