• Western blot analysis of lysates from wild type (WT) and 293T cells transfected with PADI1/PAD1 using PADI1/PAD1 Rabbit monoclonal antibody (STJ11105264) at 1:1000 dilution incubated overnight at 4 °C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 20 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 1 s.
  • Western blot analysis of lysates from Mouse ovary using PADI1/PAD1 Rabbit monoclonal antibody (STJ11105264) at 1:1400 dilution incubated overnight at 4 °C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 60 s.
  • Western blot analysis of lysates from HaCaT cells using PADI1/PAD1 Rabbit monoclonal antibody (STJ11105264) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PADI1 antibody (1-277) [S5264RM] (STJ11105264)

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STJ11105264

£146.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Protein-Arginine Deiminase Type-1 (1-277) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5264RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PADI1
Gene ID: 29943
Uniprot ID: PADI1_HUMAN
Immunogen Region: 1-277
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-277 of human PADI1/PAD1 (NP_037490.2).
Immunogen Sequence: MAPKRVVQLSLKMPTHAVCV VGVEAHVDIHSDVPKGANSF RVSGSSGVEVFMVYNRTRVK EPIGKARWPLDTDADMVVSV GTASKELKDFKVRVSYFGEQ EDQALGRSVLYLTGVDISLE VDTGRTGKVKRSQGDKKTWR WGPEGYGAILLVNCDRDNHR SAEPDLTHSWLMSLADLQDM SPMLLSCNGPDKLFDSHKLV LNVPFSDSKRVRVFCARGGN SLSDYKQVLGPQCLSYEVE
Tissue Specificity Detected in epidermal keratinocytes (at protein level). Epidermis, prostate, testis, placenta, spleen and thymus.
Function Catalyzes the deimination of arginine residues of proteins.
Protein Name Protein-Arginine Deiminase Type-1
Peptidylarginine Deiminase I
Protein-Arginine Deiminase Type I
Database Links Reactome: R-HSA-3247509
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Protein-Arginine Deiminase Type-1 antibody
Anti-Peptidylarginine Deiminase I antibody
Anti-Protein-Arginine Deiminase Type I antibody
Anti-PADI1 antibody
Anti-PAD1 antibody
Anti-PDI1 antibody

Information sourced from Uniprot.org

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