• Immunofluorescence analysis of NIH/3T3 cells using P4HA3 Rabbit polyclonal antibody (STJ115714) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using P4HA3 antibody (STJ115714) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Western blot analysis of extracts of 293T cells, using P4HA3 antibody (STJ115714) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-P4HA3 antibody (20-110) (STJ115714)

SKU:
STJ115714

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-P4HA3 (20-110) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: P4HA3
Gene ID: 283208
Uniprot ID: P4HA3_HUMAN
Immunogen Region: 20-110
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-110 of human P4HA3 (NP_878907.1).
Immunogen Sequence: DPERAAARGDTFSALTSVAR ALAPERRLLGLLRRYLRGEE ARLRDLTRFYDKVLSLHEDS TTPVANPLLAFTLIKRLQSD WRNVVHSLEAS
Tissue Specificity Highly expressed in placenta, liver and fetal skin. Weakly expressed in fetal epiphyseal cartilage, fetal liver, fibroblast, lung and skeletal muscle. Expressed also in fibrous cap of carotid atherosclerotic lesions.
Post Translational Modifications N-glycosylation plays no role in the catalytic activity.
Function Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
Protein Name Prolyl 4-Hydroxylase Subunit Alpha-3
4-Ph Alpha-3
Procollagen-Proline -2-Oxoglutarate-4-Dioxygenase Subunit Alpha-3
Database Links Reactome: R-HSA-1650814
Cellular Localisation Endoplasmic Reticulum Lumen
Alternative Antibody Names Anti-Prolyl 4-Hydroxylase Subunit Alpha-3 antibody
Anti-4-Ph Alpha-3 antibody
Anti-Procollagen-Proline -2-Oxoglutarate-4-Dioxygenase Subunit Alpha-3 antibody
Anti-P4HA3 antibody
Anti-UNQ711 antibody
Anti-PRO1374 antibody

Information sourced from Uniprot.org

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