• Immunofluorescence analysis of NIH/3T3 cells using P4HA2 Rabbit polyclonal antibody (STJ24880) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of Rat placenta, using P4HA2 antibody (STJ24880) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using P4HA2 antibody (STJ24880) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-P4HA2 antibody (239-333) (STJ24880)

SKU:
STJ24880

£112.50 - £524.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: P4HA2
Gene ID: 8974
Uniprot ID: P4HA2_HUMAN
Immunogen Region: 239-333
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 239-333 of human P4HA2 (NP_004190.1).
Immunogen Sequence: SHERAGGNLRYFEQLLEEER EKTLTNQTEAELATPEGIYE RPVDYLPERDVYESLCRGEG VKLTPRRQKRLFCRYHHGNR APQLLIAPFKEEDEW
Tissue Specificity Expressed in the heart, placenta, lung and pancreas.
Function Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
Protein Name Prolyl 4-Hydroxylase Subunit Alpha-2
4-Ph Alpha-2
Procollagen-Proline -2-Oxoglutarate-4-Dioxygenase Subunit Alpha-2
Database Links Reactome: R-HSA-1650814
Cellular Localisation Endoplasmic Reticulum Lumen
Alternative Antibody Names Anti-Prolyl 4-Hydroxylase Subunit Alpha-2 antibody
Anti-4-Ph Alpha-2 antibody
Anti-Procollagen-Proline -2-Oxoglutarate-4-Dioxygenase Subunit Alpha-2 antibody
Anti-P4HA2 antibody
Anti-UNQ290 antibody
Anti-PRO330 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance