Anti-P2RX7 antibody (STJ13100213)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from mouse P2X7 conjugated to blue carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-P2RX7 for use in IHC and WB in Mouse, Human, Rat and Marmoset samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Mouse/Human/Rat/Marmoset |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1:1000 is recommended for IHC-P and 1:2000 for WB. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20ยฐC for long term storage and refrigerated at 2-8ยฐC for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | P2RX7 |
| Gene ID | 5027 |
| Uniprot ID | P2RX7_HUMAN |
| Immunogen | A synthetic peptide from mouse P2X7 conjugated to blue carrier protein was used as the antigen. |
| Specificity | Specific for P2RX7. |
Additional Info
| Post Translational Modifications | Phosphorylation results in its inactivation. ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel. Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface. Palmitoylation prevents channel desensitization by physically anchoring the palmitoylated groups to the membrane. |
| Function | ATP-gated nonselective transmembrane cation channel that requires high millimolar concentrations of ATP for activation. Upon ATP binding, it rapidly opens to allow the influx of small cations Na(+) and Ca(2+), and the K(+) efflux. Also has the ability to form a large pore in the cell membrane, allowing the passage of large cationic molecules. In microglia, may mediate NADPH transport across the plasma membrane. In immune cells, P2RX7 acts as a molecular sensor in pathological inflammatory states by detecting and responding to high local concentrations of extracellar ATP. In microglial cells, P2RX7 activation leads to the release of pro-inflammatory cytokines, such as IL-1beta and IL-18, through the activation of the NLRP3 inflammasome and caspase-1. Cooperates with KCNK6 to activate NLRP3 inflammasome. Activates death pathways leading to apoptosis and autophagy. Activates death pathways leading to pyroptosis. Isoform B: Shows ion channel activity but no macropore function. Isoform H: Non-functional channel. Isoform J: Non-functional channel. |
| Protein Name | P2x Purinoceptor 7P2x7Atp ReceptorP2z ReceptorPurinergic Receptor |
| Database Links | Reactome: R-HSA-139853Reactome: R-HSA-418346Reactome: R-HSA-844456Reactome: R-HSA-9660826Reactome: R-HSA-9856532 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-P2x Purinoceptor 7 antibodyAnti-P2x7 antibodyAnti-Atp Receptor antibodyAnti-P2z Receptor antibodyAnti-Purinergic Receptor antibodyAnti-P2RX7 antibody |
Information sourced from Uniprot.org